Effect of thyroid status on phosphatidylinositols in rat heart

G. Jakab, E. Kiss, E. G. Kranias, I. Édes

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

The incorporation of 32Pi into phosphatidylinositols and inositol trisphosphates was studied in Langendorff-perfused hearts from hypothyroid, euthyroid and hyperthyroid rats. The hearts were perfused with modified Krebs buffer containing [32P]orthophosphate and the degree of 32P-labeling of phosphatidylinositol, phosphatidylinositol 4-monophosphate, phosphatidylinositol 4,5-bisphosphate, inositol trisphosphates and phosphatidic acid was measured. Hyperthyroidism was associated with increases in rates of rise and fall of left ventricular systolic pressure, sarcoplasmic reticular Ca2+-ATPase activity and 32P-labeling of phosphatidylinositols, inositol trisphosphates and phosphatidic acid. These measurements were significantly decreased in hypothyroid hearts. The tissue levels of inositol 1,4,5-trisphosphate isoform were found to be significantly higher in hyperthyroid hearts and lower in hypothyroid hearts than in euthyroid ones. Examination of phosphoinositide-specific phospholipase C activity in the perfused hearts revealed that hyperthyroidism was associated with an increase in the membrane-associated enzymatic activity, assayed at physiological calcium concentrations, while hypothyroidism was associated with a decrease in this activity as compared with control hearts. These findings indicate that alterations in the thyroid state of the myocardium may be associated with changes in basal phosphoinositide turnover which may contribute to alterations in myocardial contraction.

Original languageEnglish
Pages (from-to)257-262
Number of pages6
JournalCardioscience
Volume4
Issue number4
Publication statusPublished - 1993

Fingerprint

Phosphatidylinositols
Thyroid Gland
Hyperthyroidism
Phosphatidic Acids
Inositol
Myocardial Contraction
Phosphoinositide Phospholipase C
Inositol 1,4,5-Trisphosphate
Calcium-Transporting ATPases
Ventricular Pressure
Hypothyroidism
Myocardium
Buffers
Protein Isoforms
Phosphates
Blood Pressure
Calcium
Membranes

Keywords

  • hyperthyroidism
  • hypothyroidism
  • phosphatidylinositols

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine

Cite this

Jakab, G., Kiss, E., Kranias, E. G., & Édes, I. (1993). Effect of thyroid status on phosphatidylinositols in rat heart. Cardioscience, 4(4), 257-262.

Effect of thyroid status on phosphatidylinositols in rat heart. / Jakab, G.; Kiss, E.; Kranias, E. G.; Édes, I.

In: Cardioscience, Vol. 4, No. 4, 1993, p. 257-262.

Research output: Contribution to journalArticle

Jakab, G, Kiss, E, Kranias, EG & Édes, I 1993, 'Effect of thyroid status on phosphatidylinositols in rat heart', Cardioscience, vol. 4, no. 4, pp. 257-262.
Jakab, G. ; Kiss, E. ; Kranias, E. G. ; Édes, I. / Effect of thyroid status on phosphatidylinositols in rat heart. In: Cardioscience. 1993 ; Vol. 4, No. 4. pp. 257-262.
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