Effect of the preparation conditions on the surface-enhanced Raman-spectrometric identification of thin-layer-chromatographic spots

L. Kocsis, E. Horváth, J. Kristóf, R. L. Frost, A. Rédey, J. Mink

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24 Citations (Scopus)

Abstract

In situ surface enhanced Raman spectroscopic (SERS) analyses of thin layer chromatographic (TLC) spots of p-dimethyl-amino-benzylidene-rhodanine have been carried out using Nd-YAG and He-Ne lasers. With both lasers the enhancement factor was influenced by the analyte to metal (silver or gold) ratio as well. Since the enhancement factor with the Nd-YAG laser showed a stochastic distribution below 5 μg/TLC spot concentration, the NIR-SERS method can be used for in situ qualitative analyses, only. With the He-Ne laser, however, SERS spectra could be recorded in the pmol region using a concentrated silver sol. In the 1-5 μg/TLC spot region a linear relationship has been found between the SERS signal and the concentration for at least six different Raman bands. Therefore, an in situ qualitative and quantitative determination can be carried out in a single step. Copyright (C) 1999 Elsevier Science B.V.

Original languageEnglish
Pages (from-to)197-202
Number of pages6
JournalJournal of Chromatography A
Volume845
Issue number1-2
DOIs
Publication statusPublished - Jun 11 1999

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Lasers
Silver
Rhodanine
Solid-State Lasers
Polymethyl Methacrylate
Gold
Metals

Keywords

  • FT-Raman spectrometry
  • Raman microscopy
  • Surface enhancement
  • Thin-layer chromatography

ASJC Scopus subject areas

  • Analytical Chemistry

Cite this

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title = "Effect of the preparation conditions on the surface-enhanced Raman-spectrometric identification of thin-layer-chromatographic spots",
abstract = "In situ surface enhanced Raman spectroscopic (SERS) analyses of thin layer chromatographic (TLC) spots of p-dimethyl-amino-benzylidene-rhodanine have been carried out using Nd-YAG and He-Ne lasers. With both lasers the enhancement factor was influenced by the analyte to metal (silver or gold) ratio as well. Since the enhancement factor with the Nd-YAG laser showed a stochastic distribution below 5 μg/TLC spot concentration, the NIR-SERS method can be used for in situ qualitative analyses, only. With the He-Ne laser, however, SERS spectra could be recorded in the pmol region using a concentrated silver sol. In the 1-5 μg/TLC spot region a linear relationship has been found between the SERS signal and the concentration for at least six different Raman bands. Therefore, an in situ qualitative and quantitative determination can be carried out in a single step. Copyright (C) 1999 Elsevier Science B.V.",
keywords = "FT-Raman spectrometry, Raman microscopy, Surface enhancement, Thin-layer chromatography",
author = "L. Kocsis and E. Horv{\'a}th and J. Krist{\'o}f and Frost, {R. L.} and A. R{\'e}dey and J. Mink",
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TY - JOUR

T1 - Effect of the preparation conditions on the surface-enhanced Raman-spectrometric identification of thin-layer-chromatographic spots

AU - Kocsis, L.

AU - Horváth, E.

AU - Kristóf, J.

AU - Frost, R. L.

AU - Rédey, A.

AU - Mink, J.

PY - 1999/6/11

Y1 - 1999/6/11

N2 - In situ surface enhanced Raman spectroscopic (SERS) analyses of thin layer chromatographic (TLC) spots of p-dimethyl-amino-benzylidene-rhodanine have been carried out using Nd-YAG and He-Ne lasers. With both lasers the enhancement factor was influenced by the analyte to metal (silver or gold) ratio as well. Since the enhancement factor with the Nd-YAG laser showed a stochastic distribution below 5 μg/TLC spot concentration, the NIR-SERS method can be used for in situ qualitative analyses, only. With the He-Ne laser, however, SERS spectra could be recorded in the pmol region using a concentrated silver sol. In the 1-5 μg/TLC spot region a linear relationship has been found between the SERS signal and the concentration for at least six different Raman bands. Therefore, an in situ qualitative and quantitative determination can be carried out in a single step. Copyright (C) 1999 Elsevier Science B.V.

AB - In situ surface enhanced Raman spectroscopic (SERS) analyses of thin layer chromatographic (TLC) spots of p-dimethyl-amino-benzylidene-rhodanine have been carried out using Nd-YAG and He-Ne lasers. With both lasers the enhancement factor was influenced by the analyte to metal (silver or gold) ratio as well. Since the enhancement factor with the Nd-YAG laser showed a stochastic distribution below 5 μg/TLC spot concentration, the NIR-SERS method can be used for in situ qualitative analyses, only. With the He-Ne laser, however, SERS spectra could be recorded in the pmol region using a concentrated silver sol. In the 1-5 μg/TLC spot region a linear relationship has been found between the SERS signal and the concentration for at least six different Raman bands. Therefore, an in situ qualitative and quantitative determination can be carried out in a single step. Copyright (C) 1999 Elsevier Science B.V.

KW - FT-Raman spectrometry

KW - Raman microscopy

KW - Surface enhancement

KW - Thin-layer chromatography

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