Effect of the fungal mycotoxin patulin on the chromatin structure of fission yeast Schizosaccharomyces pombe

Eszter Horvath, Gabor Nagy, Melinda Turani, Eniko Balogh, Gabor Papp, Edit Pollak, I. Pócsi, M. Pesti, G. Bánfalvi

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

The fungal mycotoxin patulin is produced by several molds, especially by Aspergillus and Penicillium. The aim of this study was to clarify whether patulin causes alterations in plasma membrane permeability of Schizosaccharomyces pombe lead to cellular shrinkage charateristic to apoptosis or increases cell size indicating necrosis in cells. Transmission and scanning electronmicroscopy revealed that lower concentrations of patulin induced cellular shrinkage and blebbing, higher concentration caused expansion without cellular disruption. Large-scale morphological changes of individual cells were followed by time lapse video microscopy. Patulin caused the elongation and stickiness of cells or rounded up their shapes. To visualize chromatin structures of S. pombe nuclei upon patulin treatment, protoplasts were isolated from S. pombe and subjected to fluorescent microscopy. Chromatin changes in the presence of 50 μM patulin concentration were characterized by elongated nuclei containing sticky fibrillary chromatin and enlarged round shaped nuclei trapped at the fibrillary stage of chromatin condensation. Short (60 min) incubation of S. pombe cells in the presence of high (500 μM) patulin concentration generated patches of condensed chromatin bodies inside the nucleus and caused nuclear expansion, with the rest of chromatin remaining in fibrillary form. Longer (90 min, 500 μM) incubation resulted in fewer highly condensed chromatin patches and in nuclear fragmentation. Although, high patulin concentration increased the size of S. pombe size, it did not lead to necrotic explosion of cells, neither did the fragmented nuclei resemble apoptotic bodies that would have indicated programmed cell death. All these morphological changes and the high rate of cell survival point to rapid adaptation and mixed type of fungistatic effects.

Original languageEnglish
Pages (from-to)642-652
Number of pages11
JournalJournal of Basic Microbiology
Volume52
Issue number6
DOIs
Publication statusPublished - Dec 2012

Fingerprint

Patulin
Schizosaccharomyces
Mycotoxins
Chromatin
Video Microscopy
Explosions
Protoplasts
Penicillium
Aspergillus
Blister
Cell Size
Microscopy
Permeability
Cell Survival
Fungi
Cell Death
Necrosis
Cell Membrane
Apoptosis

Keywords

  • Cell death
  • Chromatin structure
  • Fluorescence microscopy
  • Long-term scanning
  • Protoplast
  • Scanning electronmicroscopy
  • Transmission electronmicroscopy

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology

Cite this

Effect of the fungal mycotoxin patulin on the chromatin structure of fission yeast Schizosaccharomyces pombe. / Horvath, Eszter; Nagy, Gabor; Turani, Melinda; Balogh, Eniko; Papp, Gabor; Pollak, Edit; Pócsi, I.; Pesti, M.; Bánfalvi, G.

In: Journal of Basic Microbiology, Vol. 52, No. 6, 12.2012, p. 642-652.

Research output: Contribution to journalArticle

Horvath, Eszter ; Nagy, Gabor ; Turani, Melinda ; Balogh, Eniko ; Papp, Gabor ; Pollak, Edit ; Pócsi, I. ; Pesti, M. ; Bánfalvi, G. / Effect of the fungal mycotoxin patulin on the chromatin structure of fission yeast Schizosaccharomyces pombe. In: Journal of Basic Microbiology. 2012 ; Vol. 52, No. 6. pp. 642-652.
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