Effect of the chalcone analog E,E-bis(2-hydroxybenzylidene) acetone on the 7,12-dimethylbenz[a]anthracene-induced Ha-ras gene activity in vivo

P. Perjési, I. Ember, Richard E. Bozak, Edit Nádasi, Zsuzsanna Rozmer, Timea Varjas, Ronald J. Hicks

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

The chalcone analog E,E-bis(2-hydroxybenzylidene)acetone (HBA) was found to display strong NAD(P)H:quinone reductase (NQO1) inducer potency in Hepa 1c1c7 cells. In order to determine whether this promising chemopreventive activity would extend to anticarcinogenic properties, the effect of HBA on the 7,12-dimethylbenz[a]anthracene (DMBA)-induced expression of the Ha-ras gene in isolated RNA from liver, lung, kidney, spleen, thymus, lymph nodes and bone marrow of CBA/Ca inbred mice was investigated. DMBA is a well-known chemical carcinogen, which can act as initiator by causing point mutations in certain oncogenes and tumor suppressor genes. According to the previous results, elevated Ha-ras expression has been noted even 24 h after DMBA treatment. Administration of HBA simultaneously with DMBA resulted in a decrease of the DMBA-induced Ha-ras gene expression in all the investigated tissues. This observation suggests metabolic interaction of HBA and DMBA. Administration of HBA 24 h prior to the DMBA treatment reduced the Ha-ras gene expression in all the tissues but the liver, where a slight elevation could be detected. This latter effect could be the result of a possible CYP1A inducer and pro-oxidant effects of HBA. The pro-oxidant effect of HBA can be taken into consideration based on its previously demonstrated GSH-reactivity and the present results obtained by investigation of the time-course of Fenton reaction-initiated degradation of 2-deoxyribose in the presence of HBA.

Original languageEnglish
Pages (from-to)141-146
Number of pages6
JournalIn Vivo
Volume20
Issue number1
Publication statusPublished - Jan 2006

Fingerprint

Chalcone
9,10-Dimethyl-1,2-benzanthracene
ras Genes
Genes
Gene expression
Liver
Reactive Oxygen Species
Anticarcinogenic Agents
Tissue
NAD(P)H Dehydrogenase (Quinone)
Deoxyribose
Gene Expression
Thymus
Inbred CBA Mouse
bis(2-hydroxybenzylidene)acetone
anthracene
E,E-bis(2-hydroxybenzylidene)acetone
Tumor Suppressor Genes
Oncogenes
Point Mutation

Keywords

  • 2-deoxy-D-ribose
  • 7,12-dimethylbenz[a]anthracene
  • E,E-bis(2-hydroxybenzylidene)acetone
  • Fenton-reaction
  • Ha-ras

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Effect of the chalcone analog E,E-bis(2-hydroxybenzylidene) acetone on the 7,12-dimethylbenz[a]anthracene-induced Ha-ras gene activity in vivo. / Perjési, P.; Ember, I.; Bozak, Richard E.; Nádasi, Edit; Rozmer, Zsuzsanna; Varjas, Timea; Hicks, Ronald J.

In: In Vivo, Vol. 20, No. 1, 01.2006, p. 141-146.

Research output: Contribution to journalArticle

Perjési, P. ; Ember, I. ; Bozak, Richard E. ; Nádasi, Edit ; Rozmer, Zsuzsanna ; Varjas, Timea ; Hicks, Ronald J. / Effect of the chalcone analog E,E-bis(2-hydroxybenzylidene) acetone on the 7,12-dimethylbenz[a]anthracene-induced Ha-ras gene activity in vivo. In: In Vivo. 2006 ; Vol. 20, No. 1. pp. 141-146.
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AU - Bozak, Richard E.

AU - Nádasi, Edit

AU - Rozmer, Zsuzsanna

AU - Varjas, Timea

AU - Hicks, Ronald J.

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