Effect of temperature on the separation of DNA restriction fragments in capillary gel electrophoresis

Andras Guttman, Nelson Cooke

Research output: Contribution to journalArticle

71 Citations (Scopus)

Abstract

Two different separation modes were used in high-performance capillary gel electrophoresis to study the effect of temperature on the separation of DNA restriction fragments, isoelectrostatic (use of constant applied electric field) and isorheic (use of constant current). In both instances, the migration properties and resolution of the DNA molecules were studied as a function of column temperature between 20 and 50°C. In the isoelectrostatic separation mode, the migration time and resolution decrease as temperature increases. In the isorheic separation mode, increasing the column temperature results in a maximum migration time for all of the DNA fragments and shows a maximum resolution for the lower molecular weight fragments (<200 kilodalton).

Original languageEnglish
Pages (from-to)285-294
Number of pages10
JournalJournal of Chromatography A
Volume559
Issue number1-2
DOIs
Publication statusPublished - Oct 18 1991

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Organic Chemistry

Fingerprint Dive into the research topics of 'Effect of temperature on the separation of DNA restriction fragments in capillary gel electrophoresis'. Together they form a unique fingerprint.

  • Cite this