Effect of temperature on separation efficiency in capillary gel electrophoresis

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

The effect of the separation temperature on the peak efficiency in capillary gel electrophoresis has been investigated. Low-molecular-mass pharmaceutical and carbohydrate molecules as well as high-molecular-mass biopolymers such as dsDNA and protein molecules were tested. The simple reciprocal relationship between the separation temperature and the theoretical plate numbers is distorted when the charge of the analyte changes with the temperature. Furthermore, the theoretical plates in capillary gel electrophoresis with temperature-susceptible pH buffers and complexation were found to be affected by temperature variations.

Original languageEnglish
Pages (from-to)194-198
Number of pages5
JournalTrAC - Trends in Analytical Chemistry
Volume15
Issue number5
DOIs
Publication statusPublished - May 1996

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Electrophoresis
Gels
Molecular mass
Temperature
Biopolymers
Molecules
Complexation
Buffers
Carbohydrates
Pharmaceutical Preparations
Proteins

ASJC Scopus subject areas

  • Analytical Chemistry

Cite this

Effect of temperature on separation efficiency in capillary gel electrophoresis. / Guttman, A.

In: TrAC - Trends in Analytical Chemistry, Vol. 15, No. 5, 05.1996, p. 194-198.

Research output: Contribution to journalArticle

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