Effect of temperature on retention of enantiomers of β-methyl amino acids on a teicoplanin chiral stationary phase

A. Péter, Gabriella Török, Daniel W. Armstrong, Géza Tóth, Dirk Tourwé

Research output: Contribution to journalArticle

131 Citations (Scopus)

Abstract

The isocratic retention of enantiomers of β-methyl amino acids (β-methyltyrosine, β-methylphenylalanine, β-methyltryptophan and β-methyl-1,2,3,4-tetrahydroisoquinoline-3-carboxylic acid) was studied on a teicoplanin-containing chiral stationary phase at different temperatures and at different mobile phase compositions, using the reversed-phase mode. With variation of both mobile phase composition and temperature, almost baseline separations could be achieved for all four enantiomers of sterically hindered amino acids. The retention factors and selectivity factors for the enantiomers of all investigated compounds decreased with increasing temperature. The natural logarithms of the retention factors (ln k) of the investigated compounds depended linearly on the inverse of temperature (1/T). van 't Hoff plots afforded thermodynamic parameters, such as the apparent change in enthalpy (ΔH°), the apparent change in entropy (ΔS°) and the apparent change in Gibbs free energy (ΔG°) for the transfer of analyte from the mobile to the stationary phase. The thermodynamic constants (ΔH°, ΔS°and ΔG°) were calculated in order to promote an understanding of the thermodynamic driving forces for retention in this chromatographic system. Copyright (C) 1998 Elsevier Science B.V.

Original languageEnglish
Pages (from-to)177-190
Number of pages14
JournalJournal of Chromatography A
Volume828
Issue number1-2
DOIs
Publication statusPublished - Dec 18 1998

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Teicoplanin
Enantiomers
Thermodynamics
Amino Acids
Temperature
Phase composition
Energy Transfer
Entropy
Gibbs free energy
Enthalpy
Sand

Keywords

  • Amino acids
  • Enantiomer separation
  • Methyl amino acids
  • Temperature effects
  • Thermodynamic parameters

ASJC Scopus subject areas

  • Analytical Chemistry

Cite this

Effect of temperature on retention of enantiomers of β-methyl amino acids on a teicoplanin chiral stationary phase. / Péter, A.; Török, Gabriella; Armstrong, Daniel W.; Tóth, Géza; Tourwé, Dirk.

In: Journal of Chromatography A, Vol. 828, No. 1-2, 18.12.1998, p. 177-190.

Research output: Contribution to journalArticle

Péter, A. ; Török, Gabriella ; Armstrong, Daniel W. ; Tóth, Géza ; Tourwé, Dirk. / Effect of temperature on retention of enantiomers of β-methyl amino acids on a teicoplanin chiral stationary phase. In: Journal of Chromatography A. 1998 ; Vol. 828, No. 1-2. pp. 177-190.
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