A t-2 toxin és a fermentált búzacsíra kivonatának hatása választott malacok immunvá laszára

Translated title of the contribution: Effect of T-2 toxin and fermented wheat germ extract on the immune response of weaned pigs

Szakács Árpád, Kósa Emma, T. Tuboly, Tornyos Gábor, Fodor Judit, Rajli Veronika, Kovacs Ferenc, P. Horn, Kovacs Melinda

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

The authors examined the dose dependent immunosuppressive effect of T-2 toxin, and the immunomoduiating effect of a fermented wheat germ extract (FBK) feed supplement in pigs. Weaned castrated pigs of identical genotype, and of approximately 10-12 kg body weight were divided into 9 groups (n=6 in each group). The control (C) group received toxin free diet. Diet of groups T0.5, T1.0, T1.5 and T3.0 was contaminated with 0.5,1.0,1.5 and 3.0 ppm T-2 toxin, respectively. To the toxin containing diet of groups TFB0.5, TFB1.0, TFB1.5 and TFB3.0 FBK (Immunovet-HBM®) was added in a concentration of 1 g/kg. Immune response was measured after vaccinating the animals against Aujeszky's disease (day 1 and 5). Specific and non specific in vitro cellular immune response was measured on days 0,7,14 and 21 by the lymphocyte stimulation test (LST) induced by inactivated suspension of the Aujeszky virus, and phytohaemagglutinine (PHA-P), concanavalin-A (ConA), pokeweed mitogen (PWM), respectively. Humoral immune response, e.g. specific antibody titre was measured by an Aujeszky specific ELISA. The most expressed humoral immune response was observed on day 21, although antibody levels were significantly higher in group C than in the T-2 treated animals already on day14. Immunoglobulin production in T0.5, T1.0, T1.5 and T3.0 was 44, 7,45 and 27%, respectively, compared to controls (taken as 100%) on day 21. This decrease was compensated to 74,44, 66 and 61%, respectively, using the feed additive (TFB0.5, TFB1.0, TFB1.5 and TFB3.0 groups). Changes in the cellular response due to treatments measured by LST could be observed also on days 14 and 21, on day 21 was the most expressed. The tendency was similar in case of specific (Aujeszky virus) and not specific (ConA, PHA, PWM) mitogens: 0.5-3.0 ppm T-2 toxin significantly decreased the rate of proliferation of the lymphocytes, the decrease was dose dependent and moderated by adding FBK to the diet. Cellular immune response of T-2 treated animals measured on day 21 compared to the response of the controls' (taken as 100%) concerning the specific mitogen was 78 (T0.5), 71 (T1.0), 55 (T1.5) and 48% (T3.0), which was compensated to 86 (TFB0.5), 78 (TFB1.0), 70 (TFB1.5) and 52% (TFB3.0), respectively. In case of non specific mitogens (PHA, ConA and PWM) the results were the following: 95-98 (T0.5), 92-97 (T1.0), 81-87 (T1.5), 62-77 (T3.0) in T-2 treated animals, and 102-107 (TFB0.5), 96-100 (TFB1.0), 86-93 (TFB1.5), 72-87 (TFB3.0) in the T-2+FBK groups. It could be established, that all the measured parameters were significantly and dose dependently lower in pigs fed T-2 containing diet than in the controls. The fermented wheat germ extract supplementation induced a better immune response compared to pigs exposed to T-2.

Original languageHungarian
Pages (from-to)276-282
Number of pages7
JournalMagyar Allatorvosok Lapja
Volume131
Issue number5
Publication statusPublished - 2009

Fingerprint

T-2 Toxin
T-2 toxin
wheat germ
Swine
Pokeweed Mitogens
Phytolacca americana
immune response
Diet
lymphocyte proliferation
concanavalin A
Concanavalin A
swine
Mitogens
extracts
diet
Suid herpesvirus 1
Lymphocyte Activation
Humoral Immunity
humoral immunity
Cellular Immunity

ASJC Scopus subject areas

  • veterinary(all)

Cite this

Árpád, S., Emma, K., Tuboly, T., Gábor, T., Judit, F., Veronika, R., ... Melinda, K. (2009). A t-2 toxin és a fermentált búzacsíra kivonatának hatása választott malacok immunvá laszára. Magyar Allatorvosok Lapja, 131(5), 276-282.

A t-2 toxin és a fermentált búzacsíra kivonatának hatása választott malacok immunvá laszára. / Árpád, Szakács; Emma, Kósa; Tuboly, T.; Gábor, Tornyos; Judit, Fodor; Veronika, Rajli; Ferenc, Kovacs; Horn, P.; Melinda, Kovacs.

In: Magyar Allatorvosok Lapja, Vol. 131, No. 5, 2009, p. 276-282.

Research output: Contribution to journalArticle

Árpád, S, Emma, K, Tuboly, T, Gábor, T, Judit, F, Veronika, R, Ferenc, K, Horn, P & Melinda, K 2009, 'A t-2 toxin és a fermentált búzacsíra kivonatának hatása választott malacok immunvá laszára', Magyar Allatorvosok Lapja, vol. 131, no. 5, pp. 276-282.
Árpád, Szakács ; Emma, Kósa ; Tuboly, T. ; Gábor, Tornyos ; Judit, Fodor ; Veronika, Rajli ; Ferenc, Kovacs ; Horn, P. ; Melinda, Kovacs. / A t-2 toxin és a fermentált búzacsíra kivonatának hatása választott malacok immunvá laszára. In: Magyar Allatorvosok Lapja. 2009 ; Vol. 131, No. 5. pp. 276-282.
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abstract = "The authors examined the dose dependent immunosuppressive effect of T-2 toxin, and the immunomoduiating effect of a fermented wheat germ extract (FBK) feed supplement in pigs. Weaned castrated pigs of identical genotype, and of approximately 10-12 kg body weight were divided into 9 groups (n=6 in each group). The control (C) group received toxin free diet. Diet of groups T0.5, T1.0, T1.5 and T3.0 was contaminated with 0.5,1.0,1.5 and 3.0 ppm T-2 toxin, respectively. To the toxin containing diet of groups TFB0.5, TFB1.0, TFB1.5 and TFB3.0 FBK (Immunovet-HBM{\circledR}) was added in a concentration of 1 g/kg. Immune response was measured after vaccinating the animals against Aujeszky's disease (day 1 and 5). Specific and non specific in vitro cellular immune response was measured on days 0,7,14 and 21 by the lymphocyte stimulation test (LST) induced by inactivated suspension of the Aujeszky virus, and phytohaemagglutinine (PHA-P), concanavalin-A (ConA), pokeweed mitogen (PWM), respectively. Humoral immune response, e.g. specific antibody titre was measured by an Aujeszky specific ELISA. The most expressed humoral immune response was observed on day 21, although antibody levels were significantly higher in group C than in the T-2 treated animals already on day14. Immunoglobulin production in T0.5, T1.0, T1.5 and T3.0 was 44, 7,45 and 27{\%}, respectively, compared to controls (taken as 100{\%}) on day 21. This decrease was compensated to 74,44, 66 and 61{\%}, respectively, using the feed additive (TFB0.5, TFB1.0, TFB1.5 and TFB3.0 groups). Changes in the cellular response due to treatments measured by LST could be observed also on days 14 and 21, on day 21 was the most expressed. The tendency was similar in case of specific (Aujeszky virus) and not specific (ConA, PHA, PWM) mitogens: 0.5-3.0 ppm T-2 toxin significantly decreased the rate of proliferation of the lymphocytes, the decrease was dose dependent and moderated by adding FBK to the diet. Cellular immune response of T-2 treated animals measured on day 21 compared to the response of the controls' (taken as 100{\%}) concerning the specific mitogen was 78 (T0.5), 71 (T1.0), 55 (T1.5) and 48{\%} (T3.0), which was compensated to 86 (TFB0.5), 78 (TFB1.0), 70 (TFB1.5) and 52{\%} (TFB3.0), respectively. In case of non specific mitogens (PHA, ConA and PWM) the results were the following: 95-98 (T0.5), 92-97 (T1.0), 81-87 (T1.5), 62-77 (T3.0) in T-2 treated animals, and 102-107 (TFB0.5), 96-100 (TFB1.0), 86-93 (TFB1.5), 72-87 (TFB3.0) in the T-2+FBK groups. It could be established, that all the measured parameters were significantly and dose dependently lower in pigs fed T-2 containing diet than in the controls. The fermented wheat germ extract supplementation induced a better immune response compared to pigs exposed to T-2.",
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T1 - A t-2 toxin és a fermentált búzacsíra kivonatának hatása választott malacok immunvá laszára

AU - Árpád, Szakács

AU - Emma, Kósa

AU - Tuboly, T.

AU - Gábor, Tornyos

AU - Judit, Fodor

AU - Veronika, Rajli

AU - Ferenc, Kovacs

AU - Horn, P.

AU - Melinda, Kovacs

PY - 2009

Y1 - 2009

N2 - The authors examined the dose dependent immunosuppressive effect of T-2 toxin, and the immunomoduiating effect of a fermented wheat germ extract (FBK) feed supplement in pigs. Weaned castrated pigs of identical genotype, and of approximately 10-12 kg body weight were divided into 9 groups (n=6 in each group). The control (C) group received toxin free diet. Diet of groups T0.5, T1.0, T1.5 and T3.0 was contaminated with 0.5,1.0,1.5 and 3.0 ppm T-2 toxin, respectively. To the toxin containing diet of groups TFB0.5, TFB1.0, TFB1.5 and TFB3.0 FBK (Immunovet-HBM®) was added in a concentration of 1 g/kg. Immune response was measured after vaccinating the animals against Aujeszky's disease (day 1 and 5). Specific and non specific in vitro cellular immune response was measured on days 0,7,14 and 21 by the lymphocyte stimulation test (LST) induced by inactivated suspension of the Aujeszky virus, and phytohaemagglutinine (PHA-P), concanavalin-A (ConA), pokeweed mitogen (PWM), respectively. Humoral immune response, e.g. specific antibody titre was measured by an Aujeszky specific ELISA. The most expressed humoral immune response was observed on day 21, although antibody levels were significantly higher in group C than in the T-2 treated animals already on day14. Immunoglobulin production in T0.5, T1.0, T1.5 and T3.0 was 44, 7,45 and 27%, respectively, compared to controls (taken as 100%) on day 21. This decrease was compensated to 74,44, 66 and 61%, respectively, using the feed additive (TFB0.5, TFB1.0, TFB1.5 and TFB3.0 groups). Changes in the cellular response due to treatments measured by LST could be observed also on days 14 and 21, on day 21 was the most expressed. The tendency was similar in case of specific (Aujeszky virus) and not specific (ConA, PHA, PWM) mitogens: 0.5-3.0 ppm T-2 toxin significantly decreased the rate of proliferation of the lymphocytes, the decrease was dose dependent and moderated by adding FBK to the diet. Cellular immune response of T-2 treated animals measured on day 21 compared to the response of the controls' (taken as 100%) concerning the specific mitogen was 78 (T0.5), 71 (T1.0), 55 (T1.5) and 48% (T3.0), which was compensated to 86 (TFB0.5), 78 (TFB1.0), 70 (TFB1.5) and 52% (TFB3.0), respectively. In case of non specific mitogens (PHA, ConA and PWM) the results were the following: 95-98 (T0.5), 92-97 (T1.0), 81-87 (T1.5), 62-77 (T3.0) in T-2 treated animals, and 102-107 (TFB0.5), 96-100 (TFB1.0), 86-93 (TFB1.5), 72-87 (TFB3.0) in the T-2+FBK groups. It could be established, that all the measured parameters were significantly and dose dependently lower in pigs fed T-2 containing diet than in the controls. The fermented wheat germ extract supplementation induced a better immune response compared to pigs exposed to T-2.

AB - The authors examined the dose dependent immunosuppressive effect of T-2 toxin, and the immunomoduiating effect of a fermented wheat germ extract (FBK) feed supplement in pigs. Weaned castrated pigs of identical genotype, and of approximately 10-12 kg body weight were divided into 9 groups (n=6 in each group). The control (C) group received toxin free diet. Diet of groups T0.5, T1.0, T1.5 and T3.0 was contaminated with 0.5,1.0,1.5 and 3.0 ppm T-2 toxin, respectively. To the toxin containing diet of groups TFB0.5, TFB1.0, TFB1.5 and TFB3.0 FBK (Immunovet-HBM®) was added in a concentration of 1 g/kg. Immune response was measured after vaccinating the animals against Aujeszky's disease (day 1 and 5). Specific and non specific in vitro cellular immune response was measured on days 0,7,14 and 21 by the lymphocyte stimulation test (LST) induced by inactivated suspension of the Aujeszky virus, and phytohaemagglutinine (PHA-P), concanavalin-A (ConA), pokeweed mitogen (PWM), respectively. Humoral immune response, e.g. specific antibody titre was measured by an Aujeszky specific ELISA. The most expressed humoral immune response was observed on day 21, although antibody levels were significantly higher in group C than in the T-2 treated animals already on day14. Immunoglobulin production in T0.5, T1.0, T1.5 and T3.0 was 44, 7,45 and 27%, respectively, compared to controls (taken as 100%) on day 21. This decrease was compensated to 74,44, 66 and 61%, respectively, using the feed additive (TFB0.5, TFB1.0, TFB1.5 and TFB3.0 groups). Changes in the cellular response due to treatments measured by LST could be observed also on days 14 and 21, on day 21 was the most expressed. The tendency was similar in case of specific (Aujeszky virus) and not specific (ConA, PHA, PWM) mitogens: 0.5-3.0 ppm T-2 toxin significantly decreased the rate of proliferation of the lymphocytes, the decrease was dose dependent and moderated by adding FBK to the diet. Cellular immune response of T-2 treated animals measured on day 21 compared to the response of the controls' (taken as 100%) concerning the specific mitogen was 78 (T0.5), 71 (T1.0), 55 (T1.5) and 48% (T3.0), which was compensated to 86 (TFB0.5), 78 (TFB1.0), 70 (TFB1.5) and 52% (TFB3.0), respectively. In case of non specific mitogens (PHA, ConA and PWM) the results were the following: 95-98 (T0.5), 92-97 (T1.0), 81-87 (T1.5), 62-77 (T3.0) in T-2 treated animals, and 102-107 (TFB0.5), 96-100 (TFB1.0), 86-93 (TFB1.5), 72-87 (TFB3.0) in the T-2+FBK groups. It could be established, that all the measured parameters were significantly and dose dependently lower in pigs fed T-2 containing diet than in the controls. The fermented wheat germ extract supplementation induced a better immune response compared to pigs exposed to T-2.

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