Effect of surgical and chemical sensory denervation on non-neural expression of the transient receptor potential vanilloid 1 (TRPV1) receptors in the rat

Jozsef Kun, Z. Helyes, Aniko Perkecz, Agnes Ban, B. Polgár, J. Szolcsányi, E. Pintér

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19 Citations (Scopus)

Abstract

Pretreatment with the ultrapotent capsaicin analog resiniferatoxin (RTX) has been applied as a selective pharmacological tool in inflammation and pain studies to desensitize transient receptor potential vanilloid 1 (TRPV1) receptor-expressing sensory nerve endings. The discovery of TRPV1 receptor on non-neural cells challenges systemic RTX desensitization as a method acting exclusively on a population of sensory neurons, but not on non-neural cells. Systemic RTX desensitization was used for chemical denervation and transection of the sciatic and saphenous nerves for surgical denervation in rats. Quantitative real-time PCR and immunohistochemistry were applied to investigate the presence and alterations of the TRPV1 receptor mRNA and protein following chemical and surgical denervation. We provided the first evidence for non-neural TRPV1 immunopositivity and mRNA expression in the rat dorsal paw and plantar skin as well as the oral mucosa. Neither chemical nor surgical denervation influenced the level of TRPV1 receptor mRNA and protein expression in non-neural cells of either skin regions or mucosa. Therefore, RTX and consequently capsaicin remain to be considered as selective neurotoxins for a population of primary afferent neurons.

Original languageEnglish
Pages (from-to)795-803
Number of pages9
JournalJournal of Molecular Neuroscience
Volume48
Issue number3
DOIs
Publication statusPublished - Nov 2012

Fingerprint

Denervation
Capsaicin
Sensory Receptor Cells
Messenger RNA
Afferent Neurons
Skin
Neurotoxins
Mouth Mucosa
Sciatic Nerve
Population
Real-Time Polymerase Chain Reaction
Mucous Membrane
Proteins
Immunohistochemistry
rat Trpv1 protein
vanilloid receptor subtype 1
Pharmacology
Inflammation
Pain
resiniferatoxin

Keywords

  • Denervation
  • Neural TRPV1
  • Non-neural TRPV1
  • Oral mucosa
  • Rat
  • Resiniferatoxin pretreatment
  • Skin

ASJC Scopus subject areas

  • Cellular and Molecular Neuroscience

Cite this

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abstract = "Pretreatment with the ultrapotent capsaicin analog resiniferatoxin (RTX) has been applied as a selective pharmacological tool in inflammation and pain studies to desensitize transient receptor potential vanilloid 1 (TRPV1) receptor-expressing sensory nerve endings. The discovery of TRPV1 receptor on non-neural cells challenges systemic RTX desensitization as a method acting exclusively on a population of sensory neurons, but not on non-neural cells. Systemic RTX desensitization was used for chemical denervation and transection of the sciatic and saphenous nerves for surgical denervation in rats. Quantitative real-time PCR and immunohistochemistry were applied to investigate the presence and alterations of the TRPV1 receptor mRNA and protein following chemical and surgical denervation. We provided the first evidence for non-neural TRPV1 immunopositivity and mRNA expression in the rat dorsal paw and plantar skin as well as the oral mucosa. Neither chemical nor surgical denervation influenced the level of TRPV1 receptor mRNA and protein expression in non-neural cells of either skin regions or mucosa. Therefore, RTX and consequently capsaicin remain to be considered as selective neurotoxins for a population of primary afferent neurons.",
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AU - Perkecz, Aniko

AU - Ban, Agnes

AU - Polgár, B.

AU - Szolcsányi, J.

AU - Pintér, E.

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