Effect of surface-active agents on amperometric NADH measurements with chemically modified electrodes

Géza Nagy, Imre Kapui, Lo Gorton

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

A special electrochemical pretreatment was employed in the case of finely polished glassy carbon electrode surfaces to increase the adsorption of the redox mediator Meldola blue (7-dimethylamino-1,2-benzophenoxazine). The chemically modified glassy carbon electrode holding the adsorptive mediator layer was used to measure the NADH concentration amperometrically. The interfering effect of non-ionic surfactants was studied for NADH measurements using a rotating disc electrode technique and flow injection measurements. The glassy carbon electrode response was less affected and could be regenerated much more easily than the response of a similar spectrographic graphite based electrode, studied earlier. Surface active agents can influence the apparent coverage of the surface by the mediator.

Original languageEnglish
Pages (from-to)65-73
Number of pages9
JournalAnalytica Chimica Acta
Volume305
Issue number1-3
DOIs
Publication statusPublished - Apr 20 1995

Keywords

  • Amperometry
  • Chemically modified electrodes
  • NADH

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Environmental Chemistry
  • Spectroscopy

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