Effect of succinate on mitochondrial lipid peroxidation. 1. Comparative studies on ferrous ion and ADP · Fe/NADPH-induced peroxidation

Gy Szabados, A. Andó, L. Tretter, I. Horváth

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Lipid peroxidation in isolated rat liver mitochondria, mitoplast, and mitochondrial inner membrane fragments was induced either by ferrous ions, or in an NADPH-dependent process by complexing with adenine nucleotides (ADP or ATP) iron. The Fe2+-induced lipid peroxidation is nonenzymic when inner membrane fragments are used, while the differences in the inhibitory effect of Mn2+ ions and the stimulatory effect of the ionophore A-23187 in mitochondria and inner membrane fragments suggest an enzymic mechanism for ferrous ion-induced lipid peroxidation in intact mitochondria. Contrary to this the ADP/Fe/NADPH-dependent lipid peroxidation is an enzymic process both in mitochondria and inner membrane preparations. We have shown that cytochrome P450 is involved in the ADP/Fe/NADPH-induced lipid peroxidation. Succinate, a known inhibitor of NADPH-dependent lipid peroxidation, inhibited the Fe2+-induced process also, and there was no difference in this effect when inner membrane preparations, mitochondria, or mitoplasts were used.

Original languageEnglish
Pages (from-to)21-30
Number of pages10
JournalJournal of Bioenergetics and Biomembranes
Volume19
Issue number1
DOIs
Publication statusPublished - Feb 1987

Fingerprint

Succinic Acid
NADP
Adenosine Diphosphate
Lipid Peroxidation
Ions
Mitochondria
Membranes
Adenine Nucleotides
Liver Mitochondrion
Ionophores
Calcimycin
Mitochondrial Membranes
Cytochrome P-450 Enzyme System
Iron
Adenosine Triphosphate

Keywords

  • cytochrome P
  • lipid peroxidation
  • Mitochondria
  • submitochondrial particles
  • succinate (rat liver)

ASJC Scopus subject areas

  • Physiology
  • Cell Biology

Cite this

Effect of succinate on mitochondrial lipid peroxidation. 1. Comparative studies on ferrous ion and ADP · Fe/NADPH-induced peroxidation. / Szabados, Gy; Andó, A.; Tretter, L.; Horváth, I.

In: Journal of Bioenergetics and Biomembranes, Vol. 19, No. 1, 02.1987, p. 21-30.

Research output: Contribution to journalArticle

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