Effect of stress and stress hormones on the hormone (insulin) binding of Tetrahymena

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

The unicellular Tetrahymena pyriformis GL produce, store and secrete vertebrate-like hormones. In earlier experiments the effect of different stressors on the hormone levels of Tetrahymena was studied and an elevation of these was found. In the present experiments the hormone binding was investigated, using flow cytometric method. FITC-insulin binding was elevated after concentrated (5, 10, or 20 mg ml-1) NaCl or 0.01%, 0.1%, or 0.05% formaldehyde treatment, or after thermal stress (37°C). Serotonin given together with NaCl increased and together with formaldehyde decreased the binding. Histamine always decreased the binding and insulin was indifferent. Four hours after osmotic stress, hormone binding significantly decreased and this was not influenced by hormones. However, 4 h after formaldehyde stress the binding elevated and this was further increased by repeated hormone treatments. The results show that the stress in Tetrahymena provokes an activation of its hormonal system (hormone production and binding), which is differently influenced by exogeneously given hormones.

Original languageEnglish
Pages (from-to)448-451
Number of pages4
JournalCell Biochemistry and Function
Volume27
Issue number7
DOIs
Publication statusPublished - Oct 2009

Fingerprint

Tetrahymena
Hormones
Insulin
Formaldehyde
Tetrahymena pyriformis
Osmotic Pressure
Thermal stress
Histamine
Vertebrates
Serotonin
Hot Temperature
Experiments
Chemical activation

Keywords

  • GAS
  • Hormones
  • Insulin-binding
  • Stress
  • Tetrahymena

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Clinical Biochemistry

Cite this

Effect of stress and stress hormones on the hormone (insulin) binding of Tetrahymena. / Csaba, G.; Pállinger, E.

In: Cell Biochemistry and Function, Vol. 27, No. 7, 10.2009, p. 448-451.

Research output: Contribution to journalArticle

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