Effect of streptozotocin-induced diabetes on kidney Na+/K+-ATPase

A. Ver, I. Szanto, P. Csermely, K. Kalff, E. Vegh, T. Banyasz, Z. Marcsek, T. Kovacs, J. Somogyi

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Abstract

The maximal capacity of low affinity ouabain binding sites in kidney medulla was found to be increased by 20 ± 3.8% after 2 weeks, and by 35 ± 4.5% in 4 weeks diabetes. However, in kidney cortex no similar changes could be detected. Westen blot analysis of Na+/K+-ATPase subunits in kidney medulla indicated a significant enhancement of both the α1 and β1 subunit in two and four weeks diabetic rats (α1: 35 ± 3.1, 51 ± 5.8% and β1: 31.3 ± 5.2 and 43.2 ± 6.8%, respectively). However, kidney cortex showed no significant change in any condition tested. In diabetes we could detect a significant change only in the medulla in case of the b subunit mRNA transcript, which showed 1.69 ± 0.59 and 2.89 ± 0.81 times increased in two and four weeks diabetic state, respectively. There was no change in the α1 subunit mRNA abundance. Insulin treatment of diabetic animals did not result in a complete reversal of diabetes-induced changes in ouabain binding capacity or in the amount of Na+/K+-ATPase α1 and β1 subunit protein and mRNA levels. Our data indicate a good correlation between changes in low affinity ouabain binding capacity and the level of α1 isoform in diabetic rats, and suggest an important role of the b subunit in the regulation of enzyme quantity.

Original languageEnglish
Pages (from-to)323-332
Number of pages10
JournalActa physiologica Hungarica
Volume83
Issue number4
Publication statusPublished - Dec 1 1995

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Keywords

  • Na/K-ATPase
  • kidney
  • mRNA
  • streptozotocin-induced diabetes
  • α and β subunit

ASJC Scopus subject areas

  • Physiology (medical)

Cite this

Ver, A., Szanto, I., Csermely, P., Kalff, K., Vegh, E., Banyasz, T., Marcsek, Z., Kovacs, T., & Somogyi, J. (1995). Effect of streptozotocin-induced diabetes on kidney Na+/K+-ATPase. Acta physiologica Hungarica, 83(4), 323-332.