Effect of starvation on insulin production and insulin binding in Tetrahymena

G. Csaba, P. Kovács, E. Pállinger

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Tetrahymena pyriformis GL was starved for 24 h and then the immunologically demonstrable insulin content and FITC-insulin binding were measured by flow cytometry and localization was studied by confocal microscopy. The amount of endogeneous insulin as well as FITC insulin binding, was highly significantly elevated. Glucose feeding for 30 min abolished the elevation of FITC-insulin binding. In starved cells, insulin-binding sites disappeared from the surface and FITC-insulin was bound inside the cells, within large food vacuoles. Endogeneous insulin was dispersed in the cytoplasm both in the control and starved cells and food vacuoles did not contain it. The results call attention to the stimulatory effect of starvation on insulin production in Tetrahymena, in parallel with the internal storage of insulin receptors, which points to an autocrine mechanism.

Original languageEnglish
Pages (from-to)473-477
Number of pages5
JournalCell Biochemistry and Function
Volume25
Issue number4
DOIs
Publication statusPublished - Jul 2007

Fingerprint

Tetrahymena
Starvation
Insulin
Vacuoles
Flow cytometry
Confocal microscopy
Insulin Receptor
Tetrahymena pyriformis
Food
Confocal Microscopy
Binding Sites
Flow Cytometry
Cytoplasm
Glucose
fluorescein-isothiocyanated-insulin

Keywords

  • Glucose
  • Hormone binding
  • Hormone production
  • Insulin
  • Starvation
  • Tetrahymena

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Cell Biology

Cite this

Effect of starvation on insulin production and insulin binding in Tetrahymena. / Csaba, G.; Kovács, P.; Pállinger, E.

In: Cell Biochemistry and Function, Vol. 25, No. 4, 07.2007, p. 473-477.

Research output: Contribution to journalArticle

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