Effect of soy- and whey protein-isolate supplemented diet on the redox parameters of trained mice

Dario Elia, Krisztián Stadler, Viktória Horváth, J. Jakus

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

Background: A number of clinical trials have successfully been performed using whey and/or soy proteins in the treatment of many diseases. They both have antioxidant properties, which appears to be a factor in aerobic physical performance as well. In addition, these are the most often used supplements that sportsmen take to increase their performance. Aim of the study: To investigate the effect of whey and soy protein supplementation on redox parameters in the muscle, on body weight, and body composition in swimming-trained and non-trained animals. Methods: The effect of whey and soy protein-isolate supplementation on muscle redox parameters, body weight, and body composition in trained and non-trained mice was investigated after a single exhaustive bout of exercise. Steady state free radical concentration measured using electron spin resonance (ESR) spectroscopy, reduced and oxidized glutathione ratio, thiobarbituric acid-reactive substances (TBARS), and protein carbonyl levels of the red leg muscle were measured. Results: Free radical concentrations and glutathione composition of the tissue indicated that whey protein supplementation of the regular diet was able to prevent oxidative stress regardless of training. Soy protein supplementation decreased TBARS only in the muscle of untrained animals, while training per se lowered protein damage in all investigated groups. A mixture of soy and whey protein supplementation resulted in leaner animals after training, but had no synergistic effect on either of the measured redox parameters. Conclusions: Athletes consuming these supplements could train with higher exercise intensity. The antioxidant effect of the two proteins is based on different mechanisms of action.

Original languageEnglish
Pages (from-to)259-266
Number of pages8
JournalEuropean Journal of Nutrition
Volume45
Issue number5
DOIs
Publication statusPublished - Aug 2006

Fingerprint

Soybean Proteins
whey protein isolate
protein supplements
whey protein
soy protein
Oxidation-Reduction
Diet
muscles
mice
Muscles
thiobarbituric acid-reactive substances
diet
Thiobarbituric Acid Reactive Substances
body composition
glutathione
Body Composition
exercise
Free Radicals
Glutathione
antioxidants

Keywords

  • ESR
  • Exercise
  • Glutathione
  • Oxidative stress
  • Soy protein
  • Whey protein

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Food Science

Cite this

Effect of soy- and whey protein-isolate supplemented diet on the redox parameters of trained mice. / Elia, Dario; Stadler, Krisztián; Horváth, Viktória; Jakus, J.

In: European Journal of Nutrition, Vol. 45, No. 5, 08.2006, p. 259-266.

Research output: Contribution to journalArticle

Elia, Dario ; Stadler, Krisztián ; Horváth, Viktória ; Jakus, J. / Effect of soy- and whey protein-isolate supplemented diet on the redox parameters of trained mice. In: European Journal of Nutrition. 2006 ; Vol. 45, No. 5. pp. 259-266.
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