Effect of some new cAMP analogs on cAMP-dependent protein kinase isoenzymes

Kornélia Szücs, Gyula Sági, György Vereb

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

1. 1. Ten new cAMP analogs were synthesized by replacing the purine ring with indazole, benzimidazole or benztriazole and/or their nitro and araino derivatives. 2. 2. Each analog proved effective in activating cAMP-dependent protein kinase I (PK-I) purified from rabbit skeletal muscle and cAMP-dependent protein kinase II (PK-II) from bovine heart and chasing 8-[3H]cAMP bound to regulatory subunits in the half-maximal effective concentrations of 2 × 10-8-8 × 10-6 M. 3. 3. The N-1-β-d-ribofuranosyl-indazole-3′5′-cyclophosphate(I) proved a very poor chaser and activator of both isoenzymes, but when indazole was attached at its N-2 to ribose (IV) or when its H at C-4 (equivalent to the position of amino-group in adenine) was substituted by an amino-(III) or especially nitro-group (II) its efficiency was dramatically increased. 4. 4. Analogs containing benztriazole ring proved as powerful as cAMP irrespective of the presence of substituents (VII-X). 5. 5. Benzimidazole derivatives with amino-(VI) or nitro-group (V) activated PK-II 3 and 20 times better than PK-I. 6. 6. Attaching of ribose to N-2 of indazole or benztriazole increased the affinity to PK-II 10 and 4 times, respectively. 7. 7. Chasing efficiency of cAMP analogs at half-saturating [3H]cAMP tended to correlate with activating potency only for PK-I but at saturating [3H]cAMP concentration for both isoenzymes. 8. 8. On the basis ofsynergistic activation with 8-Br-cAMP a site 2-selective binding ofnitro-benzimidazole (V) and unsubstituted benztriazole (VII) derivatives to PK-II is suggested.

Original languageEnglish
Pages (from-to)915-921
Number of pages7
JournalInternational Journal of Biochemistry
Volume24
Issue number6
DOIs
Publication statusPublished - Jun 1992

ASJC Scopus subject areas

  • Biochemistry

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