Effect of solution conformation on antibody recognition of a protein core epitope from gastrointestinal mucin (MUC2)

K. Uray, Judit Kajtár, E. Vass, Michael R. Price, M. Hollósi, F. Hudecz

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Antibody recognition of the tandem repeat unit of MUC2 glycoprotein was investigated. To clarify the role of secondary structure, the immunoreactivity and conformation of overlapping and truncated peptides were investigated. For this several MUC2 peptides have been synthesized and their secondary structure has been analyzed by circular dichroism and Fourier transform infrared spectroscopical methods. For the binding studies a MUC2 mucin protein core-specific monoclonal antibody was used in competition RIA experiments. The minimal size peptide functioning as epitope was peptide 18PTGTQ22. Within the immunodominant 18TPTPTPTGTQTPTT26 region we found that all peptides recognized by the 996 monoclonal antibody adopted β- turns secondary structure. Peptides 15TPTPTGTQ22 and 16PTPTGTQ22, containing the most prominent β-turn(s), had the strongest immunoreactivity. It was also observed that peptides with Pro on their N-termini (16PTPTGTQ22, 18PTGTQ22) adopt a different type of β-turn in TFE than peptides with Thr at their N-terminal. Based on the antibody binding, molecular dynamics calculations, and secondary structure analysis, we propose a model for the epitope structure of the MUC2 mucin tandem repeat.

Original languageEnglish
Pages (from-to)65-74
Number of pages10
JournalArchives of Biochemistry and Biophysics
Volume361
Issue number1
DOIs
Publication statusPublished - Jan 1 1999

Fingerprint

Mucins
Conformations
Epitopes
Peptides
Antibodies
Proteins
Tandem Repeat Sequences
Monoclonal Antibodies
Immunodominant Epitopes
Polytetrafluoroethylene
Dichroism
Fourier Analysis
Molecular Dynamics Simulation
Circular Dichroism
Molecular dynamics
Glycoproteins
Fourier transforms
Infrared radiation

Keywords

  • CD
  • FT- IR
  • Immunochemistry
  • MUC2 mucin
  • Secondary structure of synthetic peptides

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Effect of solution conformation on antibody recognition of a protein core epitope from gastrointestinal mucin (MUC2). / Uray, K.; Kajtár, Judit; Vass, E.; Price, Michael R.; Hollósi, M.; Hudecz, F.

In: Archives of Biochemistry and Biophysics, Vol. 361, No. 1, 01.01.1999, p. 65-74.

Research output: Contribution to journalArticle

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