Effect of singlet oxygen generating substances on the ascorbic acid and glutathione content in pea leaves

G. Gullner, Alan D. Dodge

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Ascorbate and glutathione levels were investigated in pea leaf discs exposed to various singlet oxygen generating compounds: eosin, rose bengal, monuron, acifluorfen and 5-amino-levulinic acid (ALA). The cellular level of the major antioxidant ascorbate was markedly decreased by the herbicides monuron, acifluorfen and ALA (in light-dependent reactions), as well as by the xanthene dyes eosin and rose bengal (independently of light). No significant accumulation of dehydroascorbate could be observed in any treatments. In contrast to ascorbate, the foliar glutathione levels were considerably increased by subtoxic or slightly toxic concentrations of eosin, rose bengal, acifluorfen and ALA in a light-]dependent manner. Monuron treatments led to unchanged or decreasing glutathione contents. The activities of three antioxidative enzymes (ascorbate peroxidase, glutathione reductase and glutathione S-transferase) were also induced by eosin in light- dependent reactions. (C) 2000 Published by Elsevier Science Ireland Ltd.

Original languageEnglish
Pages (from-to)127-133
Number of pages7
JournalPlant Science
Volume154
Issue number2
DOIs
Publication statusPublished - May 29 2000

Fingerprint

Singlet Oxygen
singlet oxygen
monuron
Peas
acifluorfen
Eosine Yellowish-(YS)
Rose Bengal
aminolevulinic acid
Ascorbic Acid
Glutathione
glutathione
peas
ascorbic acid
Light
leaves
Xanthenes
Oxygen Compounds
Ascorbate Peroxidases
Glutathione Reductase
Poisons

Keywords

  • 5-Aminolevulinic acid
  • Acifluorfen
  • Ascorbate
  • Glutathione
  • Monuron
  • Xanthene dye

ASJC Scopus subject areas

  • Plant Science
  • Biochemistry
  • Biotechnology

Cite this

Effect of singlet oxygen generating substances on the ascorbic acid and glutathione content in pea leaves. / Gullner, G.; Dodge, Alan D.

In: Plant Science, Vol. 154, No. 2, 29.05.2000, p. 127-133.

Research output: Contribution to journalArticle

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