Effect of simulated microgravity on the production of IL-12 by PBMCs

Ágnes Bakos, Andrea Várkonyi, J. Mináróvits, László Bátkai

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

During space flight immunity is altered. This phenomenon is partly due to the microgravity condition itself. Our earlier space experiments (INTERFERON) indicated that microgravity has a significant effect at the cellular level. In our subsequent terrestrial studies we applied the Rotating Cell Culture System (RCCS) developed by NASA to mimick microgravity on ground. Previously we reported that human peripheral blood mononuclear cells (PBMCs) respond to simulated microgravity conditions with elevated tumor necrosis factor-alpha (TNF-α) production. We extended our investigations to the production of interleukin (IL)-12 under modelled microgravity conditions by separated PBMCs. In simulated microgravity we found significantly elevated level of secreted IL-12 compared to static, standard tissue culture conditions. Following a maximum of TNF-α production at 24 hours, the peak of IL-12 production was observed at 48 hours after the start of the experiment. Our results suggest that simulated microgravity favors the establishment of a Th1 type cytokine response.

Original languageEnglish
Title of host publicationEuropean Space Agency, (Special Publication) ESA SP
Pages335-336
Number of pages2
Edition501
Publication statusPublished - Sep 2002
EventProceedings of the European Symposium on Life in Space for Life on Earth - Stockholm, Sweden
Duration: Jun 2 2002Jun 7 2002

Other

OtherProceedings of the European Symposium on Life in Space for Life on Earth
CountrySweden
CityStockholm
Period6/2/026/7/02

Fingerprint

Microgravity
Blood
Tissue culture
Space flight
Cell culture
NASA
Experiments

ASJC Scopus subject areas

  • Aerospace Engineering

Cite this

Bakos, Á., Várkonyi, A., Mináróvits, J., & Bátkai, L. (2002). Effect of simulated microgravity on the production of IL-12 by PBMCs. In European Space Agency, (Special Publication) ESA SP (501 ed., pp. 335-336)

Effect of simulated microgravity on the production of IL-12 by PBMCs. / Bakos, Ágnes; Várkonyi, Andrea; Mináróvits, J.; Bátkai, László.

European Space Agency, (Special Publication) ESA SP. 501. ed. 2002. p. 335-336.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Bakos, Á, Várkonyi, A, Mináróvits, J & Bátkai, L 2002, Effect of simulated microgravity on the production of IL-12 by PBMCs. in European Space Agency, (Special Publication) ESA SP. 501 edn, pp. 335-336, Proceedings of the European Symposium on Life in Space for Life on Earth, Stockholm, Sweden, 6/2/02.
Bakos Á, Várkonyi A, Mináróvits J, Bátkai L. Effect of simulated microgravity on the production of IL-12 by PBMCs. In European Space Agency, (Special Publication) ESA SP. 501 ed. 2002. p. 335-336
Bakos, Ágnes ; Várkonyi, Andrea ; Mináróvits, J. ; Bátkai, László. / Effect of simulated microgravity on the production of IL-12 by PBMCs. European Space Agency, (Special Publication) ESA SP. 501. ed. 2002. pp. 335-336
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