Effect of short-term sterigmatocystin exposure on lipid peroxidation and glutathione redox system and expression of glutathione redox system regulatory genes in common carp liver

Benjamin Kövesi, Csilla Pelyhe, Erika Zándoki, M. Mézes, K. Balogh

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Sterigmatocystin (STC) is structurally close to the mycotoxin aflatoxin B 1 as it shares its biosynthetic pathway with aflatoxins. The purpose of the present study was to investigate the short-term (24 h) effects of STC contaminated diet at different doses (1 mg, 2 mg and 4 mg STC kg −1 feed) in one year old common carp juveniles. Liver samples were taken in 8-h intervals. The markers of the lipid peroxidation showed moderate changes after the application of sterigmatocystin-contaminated diet, significant elevations were only observed in the lowest applied dose group of sterigmatocystin after 16 h of exposure. Reduced glutathione content showed higher levels than control group after 16 h of exposure as effect of low dose of sterigmatocystin. Glutathione peroxidase (GPX4) activity was lower than control in the group treated with 2 mg STC kg −1 feed after 24 h of exposure. Gene expression measurements of keap1, nrf2, gpx4a, gpx4b and gss genes revealed a dual response. Down-regulation or near control values were observed 8 h after exposure which was followed by an induction 16 and 24 h after exposure. In case of gsr, gene expression values returned to control levels by the 24th hour. In summary, these results suggest that lower doses of STC caused oxidative stress earlier than higher doses, which efficiently activated the Keap1-Nrf2 pathway, while higher doses revealed long-drawn activation of this pathway.

Original languageEnglish
Pages (from-to)50-56
Number of pages7
JournalToxicon
Volume161
DOIs
Publication statusPublished - Apr 1 2019

Fingerprint

Sterigmatocystin
Carps
Regulator Genes
Liver
Lipid Peroxidation
Oxidation-Reduction
Glutathione
Genes
Lipids
Level control
phospholipid-hydroperoxide glutathione peroxidase
Nutrition
Gene expression
Diet
Gene Expression
Control Groups
Aflatoxin B1
Aflatoxins
Oxidative stress
Mycotoxins

Keywords

  • Antioxidant defence system
  • Common carp
  • Gene expression
  • Glutathione peroxidase
  • Sterigmatocystin

ASJC Scopus subject areas

  • Toxicology

Cite this

Effect of short-term sterigmatocystin exposure on lipid peroxidation and glutathione redox system and expression of glutathione redox system regulatory genes in common carp liver. / Kövesi, Benjamin; Pelyhe, Csilla; Zándoki, Erika; Mézes, M.; Balogh, K.

In: Toxicon, Vol. 161, 01.04.2019, p. 50-56.

Research output: Contribution to journalArticle

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AU - Balogh, K.

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