Effect of sample handling on alkaloid and mineral content of aqueous extracts of greater celandine (Chelidonium majus L.)

Mária Then, Klára Szentmihályi, Ágnes Sárközi, Vendel Illés, Eszter Forgács

Research output: Contribution to journalArticle

27 Citations (Scopus)


The authors examined the extraction of alkaloids from the greater celandine (Chelidonium majus L.) by different methods (traditional pressing and tea making, microwave and supercritical fluid extraction). The extractants were water and propylene glycol. For comparison of the extraction methods, the yield was evaluated according to total alkaloid content measured by spectroscopy. The highest alkaloid yield was obtained by microwave extraction and by making tea. Distribution of the components was studied by thin-layer chromatography and densitometry. The concentration and the ratio of alkaloid components in extracts are significantly different depending on the extraction method. The solution obtained by supercritical fluid extraction contains coptisine and chelidonine, while berberine could be obtained by microwave extraction only. Extracts with high coptisine content were obtained by supercritical fluid extraction, followed by pressing and microwave extraction. Mineral element content of the drug and extracts was also determined by inductively coupled plasma atomic emission spectrometry. Element content (Na, Ca, Fe) was found to be highest in microwave extracts. Copyright (C) 2000 Elsevier Science B.V.

Original languageEnglish
Pages (from-to)69-74
Number of pages6
JournalJournal of Chromatography A
Issue number1-2
Publication statusPublished - Aug 11 2000


  • Alkaloids
  • Chelidonine
  • Chelidonium majus
  • Coptisine
  • Extraction methods
  • Food analysis
  • Metal cations
  • Tea

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Organic Chemistry

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