Effect of pressure on solute capacity factor in HPLC using a non-porous stationary phase

R. Ohmacht, B. Boros

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Abstract

The pressure applied in liquid chromatography (LC) can influence the capacity factor of analytes. Using commercial equipment, column (33 x 4.6 mm) and 1.5 μm non-porous, tetradecyl (C14) coated silica particles, a moderate but significant change in the capacity factor was observed with increasing pressure. Depending on the experimental conditions the relationship was found to be either linear or non-linear. In the latter case the retention increased at first, but later tended to decrease at still higher pressure. The results direct attention to the role of the pressure (and hence of flow rate and heat of friction) in determining capacity factors.

Original languageEnglish
Pages (from-to)S205-S210
JournalChromatographia
Volume51
Issue number1
Publication statusPublished - Jan 1 2000

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Keywords

  • Column liquid chromatography
  • Non-porous stationary phases
  • Pressure influence of capacity factor
  • Solute retention

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Clinical Biochemistry
  • Organic Chemistry

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