Effect of precultivation conditions on colicin susceptibility in Escherichia coli

I. Gadó, J. Pászti, V. G. László

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Abstract

The effect of 20 colicins and cloacin was studied after various precultivations. Nutrient agar supplemented with subinhibitory concentration of EDTA used for precultivation or elevating the growth-temperature of the inoculum from 37°C to 42°C increased the susceptibility of wild-type (smooth) Escherichia coli strains to the inhibitory action of some colicins. There were great differences among the colicins in respect to these effects. In case of rough mutants, their sensitivities did not change or eventually decrease after EDTA or heat pretreatment. The LPS pattern in SDS-PAGE of smooth cells grown in EDTA-containing nutrient medium changed in some degree towards the rough character. In case of precultivation at 42°C this change was less considerable. It is supposed that both factors applied during precultivation have influence on colicin sensitivity by means of the change of receptor activity caused by LPS modification.

Original languageEnglish
Pages (from-to)100-106
Number of pages7
JournalJournal of Chemotherapy
Volume7
Issue number2
DOIs
Publication statusPublished - Jan 1 1995

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Keywords

  • Colicin
  • EDTA
  • Heat treatment
  • Sensitivity

ASJC Scopus subject areas

  • Oncology
  • Pharmacology
  • Pharmacology (medical)
  • Infectious Diseases

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