Effect of phosphatidylglycerol depletion on the surface electric properties and the fluorescence emission of thylakoid membranes

Emilia L. Apostolova, Ildikó Domonkos, Anelia G. Dobrikova, Anna Sallai, Balázs Bogos, Hajime Wada, Z. Gombos, Stefka G. Taneva

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

To explore the possible effect of phosphatidylglycerol (PG) on the surface electric properties and chlorophyll fluorescence characteristics we used electric light scattering technique and 77 K chlorophyll fluorescence of thylakoid membranes from a cyanobacterium, Synechocystis PCC6803 (wild type) and its pgsA mutant defective in PG synthesis. We found a strong decrease in the permanent and induced electric dipole moments of the mutant thylakoids, following long-term PG depletion parallel with a decrease of the emission peak from PSI and an increase of the emission peak from PSII. Partial recovery of the electric state of thylakoid membranes was observed at re-addition of PG to the mutant cells depleted of PG for 21days. This change in the electric dipole moments is probably due to a decrease in PG content and progressive structural alterations in the macroorganization of the photosynthetic complexes induced by PG deprivation. Our results suggest that the depletion of a lipid, which carries a negative charge, despite its small contribution to the overall lipid content, significantly perturbs the surface charge of the membranes. These changes are related with the chlorophyll fluorescence emission ratios of two photosystems and may partly explain our earlier results concerning the PG requirement for the function and assembly of photosystems I and II reaction centers.

Original languageEnglish
Pages (from-to)51-57
Number of pages7
JournalJournal of Photochemistry and Photobiology, B: Biology
Volume91
Issue number1
DOIs
Publication statusPublished - Apr 25 2008

Fingerprint

electrical properties
Phosphatidylglycerols
Thylakoids
Surface Properties
phosphatidylglycerols
chlorophylls
Chlorophyll
thylakoids
Electric dipole moments
depletion
Electric properties
Fluorescence
fluorescence
electric moments
membranes
Membranes
Lipids
electric dipoles
lipids
dipole moments

Keywords

  • Chlorophyll fluorescence
  • Electric light scattering
  • pgsA mutant
  • Phosphatidylglycerol
  • Synechocystis PCC6803
  • Thylakoid membranes

ASJC Scopus subject areas

  • Plant Science
  • Bioengineering
  • Physical and Theoretical Chemistry

Cite this

Effect of phosphatidylglycerol depletion on the surface electric properties and the fluorescence emission of thylakoid membranes. / Apostolova, Emilia L.; Domonkos, Ildikó; Dobrikova, Anelia G.; Sallai, Anna; Bogos, Balázs; Wada, Hajime; Gombos, Z.; Taneva, Stefka G.

In: Journal of Photochemistry and Photobiology, B: Biology, Vol. 91, No. 1, 25.04.2008, p. 51-57.

Research output: Contribution to journalArticle

Apostolova, Emilia L. ; Domonkos, Ildikó ; Dobrikova, Anelia G. ; Sallai, Anna ; Bogos, Balázs ; Wada, Hajime ; Gombos, Z. ; Taneva, Stefka G. / Effect of phosphatidylglycerol depletion on the surface electric properties and the fluorescence emission of thylakoid membranes. In: Journal of Photochemistry and Photobiology, B: Biology. 2008 ; Vol. 91, No. 1. pp. 51-57.
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