Effect of phorbol 12‐myristate 13‐acetate (PMA) on the phosphoinositol (PI) system in Tetrahymena. Study of the 32P incorporation and breakdown of phospholipids

P. Kovács, G. Csaba

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11 Citations (Scopus)

Abstract

Phorbol 12‐myristate 13‐acetate (PMA) treatment elicited an increased 32P incorporation into phospholipids namely phosphatadyl‐inositol (PI); phosphatidyl‐inositol‐4‐phosphate (PIP); phosphatidyl‐inositol‐4,5‐bis‐phosphate (PIP2); phosphatidyl‐acid (PA); phosphatidyl‐choline (PC) and phosphatidyl‐ethanolamine (PE) particularly at the 20–30th min after treatment. The ratio of members of the phosphoinositol system, especially PIP and PI, related to the total phospholipid content was increased. PMA (2 × 10−7 M) was the most effective of the three concentrations tested. The results call attention to the presence of a working phosphoinositol system in Protozoa.

Original languageEnglish
Pages (from-to)85-89
Number of pages5
JournalCell biochemistry and function
Volume13
Issue number2
DOIs
Publication statusPublished - Jun 1995

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Keywords

  • Phosphoinositol system
  • Tetrahymena
  • phorbol ester
  • second messengers

ASJC Scopus subject areas

  • Biochemistry
  • Clinical Biochemistry
  • Cell Biology

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