Effect of phalloidin on the skeletal muscle ADP-actin filaments

Réka Dudás, Tünde Kupi, Andrea Vig, J. Orbán, D. Lőrinczy

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

The effect of phalloidin on the thermal stability of skeletal actin filaments polymerized from ADP-binding monomers was investigated with the method of differential scanning calorimetry. Phalloidin shifted the melting temperature of the ADP-F-actin from 59.1±1.0 to 80.0±1.2°C. The stabilizing effect of phalloidin propagated cooperatively along the filament. The cooperativity factor according to the applied model was 1.07±0.11. With these measurements it was possible to demonstrate that the binding of phalloidin has lower influence on the adjacent protomers in ADP- (k=1) than in ATP-actin filaments (k=3).

Original languageEnglish
Pages (from-to)709-712
Number of pages4
JournalJournal of Thermal Analysis and Calorimetry
Volume95
Issue number3
DOIs
Publication statusPublished - Mar 2009

Fingerprint

Phalloidine
adenosine diphosphate
skeletal muscle
Muscle
filaments
Adenosine Diphosphate
Actins
adenosine triphosphate
Protein Subunits
thermal stability
heat measurement
monomers
melting
Melting point
Differential scanning calorimetry
Thermodynamic stability
scanning
Monomers
ADP-G-actin
temperature

Keywords

  • ADP-actin filament
  • Calorimetry
  • Phalloidin
  • Stability
  • Thermodynamics

ASJC Scopus subject areas

  • Physical and Theoretical Chemistry
  • Condensed Matter Physics

Cite this

Effect of phalloidin on the skeletal muscle ADP-actin filaments. / Dudás, Réka; Kupi, Tünde; Vig, Andrea; Orbán, J.; Lőrinczy, D.

In: Journal of Thermal Analysis and Calorimetry, Vol. 95, No. 3, 03.2009, p. 709-712.

Research output: Contribution to journalArticle

Dudás, Réka ; Kupi, Tünde ; Vig, Andrea ; Orbán, J. ; Lőrinczy, D. / Effect of phalloidin on the skeletal muscle ADP-actin filaments. In: Journal of Thermal Analysis and Calorimetry. 2009 ; Vol. 95, No. 3. pp. 709-712.
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