Effect of phalloidin on filaments polymerized from heart muscle ADP-actin monomers

Andrea Vig, Réka Dudás, Tünde Kupi, J. Orbán, G. Hild, D. Lőrinczy, M. Nyitrai

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

The effect of phalloidin on filaments polymerized from ADP-actin monomers of the heart muscle was investigated with differential scanning calorimetry. Heart muscle contains α-skeletal and α-cardiac actin isoforms. In the absence of phalloidin the melting temperature was 55°C for the α-cardiac actin isoform and 58°C for the α-skeletal one when the filaments were generated from ADP-actin monomers. After the binding of phalloidin the melting temperature was isoform independent (85.5°C). We concluded that phalloidin stabilized the actin filaments of α-skeletal and α-cardiac actin isoforms to the same extent when they were polymerized from ADP-actin monomers.

Original languageEnglish
Pages (from-to)721-725
Number of pages5
JournalJournal of Thermal Analysis and Calorimetry
Volume95
Issue number3
DOIs
Publication statusPublished - Mar 2009

Keywords

  • Calorimetry
  • Cardiac ADP-F-actin
  • Isoform
  • Phalloidin
  • Thermodynamics

ASJC Scopus subject areas

  • Condensed Matter Physics
  • Physical and Theoretical Chemistry

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