Effect of pH on cellulase production of Trichoderma reesei RUT C30

Tamás Juhász, Zsolt Szengyel, Nóra Szijártó, Kati Réczey

Research output: Contribution to journalArticle

47 Citations (Scopus)


Currently, the high market price of cellulases prohibits commercialization of the lignocellulosics-to-fuel ethanol process, which utilizes enzymes for saccharification of cellulose. For this reason research aimed at understanding and improving cellulase production is still a hot topic in cellulase research. Trichoderma reesei RUT C30 is known to be one of the best hyper producing cellulolytic fungi, which makes it an ideal test organism for research. New findings could be adopted for industrial strains in the hope of improving enzyme yields, which in turn may result in lower market price of cellulases, thus making fuel ethanol more cost competitive with fossil fuels. Being one of the factors affecting the growth and cellulase production of T. reesei, the pH of cultivation is of major interest. In the present work, numerous pH-controlling strategies were compared both in shake-flask cultures and in a fermentor. Application of various buffer systems in shake-flask experiments was also tested. Although application of buffers resulted in slightly lower cellulase activity than that obtained in non-buffered medium, β-glucosidase production was increased greatly.

Original languageEnglish
Pages (from-to)201-211
Number of pages11
JournalApplied Biochemistry and Biotechnology - Part A Enzyme Engineering and Biotechnology
Issue number1-3
Publication statusPublished - Mar 1 2004


  • Cellulase production
  • Fermentor
  • Shake flask
  • Trichoderma reesei RUT C30
  • pH profiling
  • β-glucosidase

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology

Fingerprint Dive into the research topics of 'Effect of pH on cellulase production of Trichoderma reesei RUT C30'. Together they form a unique fingerprint.

  • Cite this