Effect of PACAP on LH release studied by cell immunoblot assay depends on the gender, on the time of day and in female rats on the day of the estrous cycle

E. Szabó, Á Nemeskéri, A. Arimura, K. Köves

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

We have previously demonstrated that pituitary adenylate cyclase activating polypeptide (PACAP) can be released from cultured rat anterior pituitary cells and when added to the medium in physiological concentration it releases LH from individual gonadotropes. In the present work, we studied whether the release of PACAP and the responsiveness of LH cells to PACAP depend on the gender, on the time of day when the animals were sacrificed, and in females on the stage of the estrous cycle. Anterior pituitary cells were cultured on nitrocellulose membrane. We found that the number of PACAP releasing cells was higher in proestrous than in diestrous female or in male rats and their number was always higher in the evening than at the other times. The effect of PACAP on LH cells was stimulatory in the morning of proestrus and diestrus. In proestrous rats, PACAP did not influence LH release in the afternoon or the evening, but in diestrous rats it decreased it in the afternoon and the evening. In males, there was a decrease of LH due to PACAP treatment at 10 and 20 h; however, PACAP did not influence LH at 16 h. It was concluded that in vivo PACAP might be involved in the circadian and episodic release of LH at pituitary level.

Original languageEnglish
Pages (from-to)139-145
Number of pages7
JournalRegulatory Peptides
Volume123
Issue number1-3 SPEC. ISS.
DOIs
Publication statusPublished - Dec 15 2004

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Pituitary Adenylate Cyclase-Activating Polypeptide
Estrous Cycle
Rats
Assays
Gonadotrophs
Diestrus
Proestrus
Collodion
Cultured Cells
Animals
Membranes

Keywords

  • Cell culturing
  • Image analysis
  • Immunocytochemistry

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology
  • Physiology
  • Neuroscience(all)

Cite this

Effect of PACAP on LH release studied by cell immunoblot assay depends on the gender, on the time of day and in female rats on the day of the estrous cycle. / Szabó, E.; Nemeskéri, Á; Arimura, A.; Köves, K.

In: Regulatory Peptides, Vol. 123, No. 1-3 SPEC. ISS., 15.12.2004, p. 139-145.

Research output: Contribution to journalArticle

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