Effect of oxygen free radicals on myosin in muscle fibres

Franciska Könczöl, D. Lőrinczy, J. Belágyi

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Experiments were performed on glycerol-extracted muscle fibres prepared from psoas muscle of rabbit in the presence of hydroxyl free radical generating system. Short irradiation of spin-labelled muscle fibres by UV light showed the interaction of probe molecules with oxygen free radicals. The intensity of the EPR signal from maleimide or isothiocyanate spin labels attached to the essential thiol groups decreased following irradiation. Oxygen free radicals affected the rate constant of the transition AM.ADP.V(i) → AM.ADP in the ATP hydrolysis cycle. It was found that the essential -SH groups of myosin were involved in the oxidation of sulphydryls by Ce(IV). Ce(IV) complexed to nitrilotriacetic acid in the presence of spin trap produced long-lived free radicals located partly on SH-1 sulphydryls.

Original languageEnglish
Pages (from-to)341-344
Number of pages4
JournalFEBS Letters
Volume427
Issue number3
DOIs
Publication statusPublished - May 15 1998

Fingerprint

Myosins
Free Radicals
Muscle
Reactive Oxygen Species
Oxygen
Muscles
Fibers
Adenosine Diphosphate
Nitrilotriacetic Acid
Irradiation
Psoas Muscles
Spin Labels
Ultraviolet Rays
Sulfhydryl Compounds
Ultraviolet radiation
Hydroxyl Radical
Glycerol
Paramagnetic resonance
Hydrolysis
Rate constants

Keywords

  • Myosin
  • Oxygen free radical
  • Spin-trapping

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Effect of oxygen free radicals on myosin in muscle fibres. / Könczöl, Franciska; Lőrinczy, D.; Belágyi, J.

In: FEBS Letters, Vol. 427, No. 3, 15.05.1998, p. 341-344.

Research output: Contribution to journalArticle

Könczöl, Franciska ; Lőrinczy, D. ; Belágyi, J. / Effect of oxygen free radicals on myosin in muscle fibres. In: FEBS Letters. 1998 ; Vol. 427, No. 3. pp. 341-344.
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