Effect of oxidative stress on the junctional proteins of cultured cerebral endothelial cells

I. Krizbai, Hannelore Bauer, Nicolaus Bresgen, Peter M. Eckl, Attila Farkas, Erzsébet Szatmári, Andreas Traweger, Katarzyna Wejksza, Hans Christian Bauer

Research output: Contribution to journalArticle

76 Citations (Scopus)

Abstract

1. There is increasing evidence that the cerebral endothelium and the blood-brain barrier (BBB) plays an important role in the oxidative stress-induced brain damage. The aim of the present study was to investigate the role of interendothelial junctional proteins in the BBB permeability increase induced by oxidative stress. 2. For the experiments, we have used cultured cerebral endothelial cells exposed to hypoxia/reoxygenation or treated with the redox cycling quinone 2,3-Dimethoxy-1,4-naphthoquinone (DMNQ) in the presence or absence of glucose. The expression of junctional proteins and activation of mitogen activated protein kinases (MAPK) was followed by Western-blotting, the interaction of junctional proteins was investigated using coimmunoprecipitation. 3. Oxidative stress induces a downregulation of the tight junction protein occludin expression which is more pronounced in the absence of glucose. Furthermore, oxidative stress leads to disruption of the cadherin-β-catenin complex and an activation of extracellular signal-regulated kinase (ERK1/2), which is more intense in the absence of glucose. 4. We have shown that one of the causes of the BBB breakdown is probably the structural alteration of the junctional complex caused by oxidative stress, a process in which ERK1/2 may play an important role.

Original languageEnglish
Pages (from-to)129-139
Number of pages11
JournalCellular and Molecular Neurobiology
Volume25
Issue number1
DOIs
Publication statusPublished - Feb 2005

Fingerprint

Oxidative stress
Endothelial cells
Oxidative Stress
Endothelial Cells
Blood-Brain Barrier
Proteins
Glucose
Chemical activation
Occludin
Tight Junction Proteins
Catenins
Mitogen-Activated Protein Kinase 1
Extracellular Signal-Regulated MAP Kinases
Cadherins
Mitogen-Activated Protein Kinases
Oxidation-Reduction
Endothelium
Permeability
Brain
Down-Regulation

Keywords

  • Cerebral endothelial cells
  • MAPK
  • Occludin
  • Oxidative stress
  • tight junction

ASJC Scopus subject areas

  • Neuroscience(all)
  • Clinical Biochemistry
  • Cell Biology
  • Genetics

Cite this

Effect of oxidative stress on the junctional proteins of cultured cerebral endothelial cells. / Krizbai, I.; Bauer, Hannelore; Bresgen, Nicolaus; Eckl, Peter M.; Farkas, Attila; Szatmári, Erzsébet; Traweger, Andreas; Wejksza, Katarzyna; Bauer, Hans Christian.

In: Cellular and Molecular Neurobiology, Vol. 25, No. 1, 02.2005, p. 129-139.

Research output: Contribution to journalArticle

Krizbai, I, Bauer, H, Bresgen, N, Eckl, PM, Farkas, A, Szatmári, E, Traweger, A, Wejksza, K & Bauer, HC 2005, 'Effect of oxidative stress on the junctional proteins of cultured cerebral endothelial cells', Cellular and Molecular Neurobiology, vol. 25, no. 1, pp. 129-139. https://doi.org/10.1007/s10571-004-1378-7
Krizbai, I. ; Bauer, Hannelore ; Bresgen, Nicolaus ; Eckl, Peter M. ; Farkas, Attila ; Szatmári, Erzsébet ; Traweger, Andreas ; Wejksza, Katarzyna ; Bauer, Hans Christian. / Effect of oxidative stress on the junctional proteins of cultured cerebral endothelial cells. In: Cellular and Molecular Neurobiology. 2005 ; Vol. 25, No. 1. pp. 129-139.
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