N-ethyl-N-/2-methyl-2-propenyl/, N-butyl-N-ethyl and N, N-dipropyl derivatives of 2, 6-dinitro-4-/trifluoromethyl/ /-aniline were separated on alumina TLC layers with n-hexane eluent applying one, two and three developments. The second development enhanced considerably the resolution and also the detection limit. The third development had a negligible effect on the resolution, however the detection limit was further increased. Due to the lateral diffusion the half width of peaks decreased linearly with the number of developments. The data proved that the third development had no additional advantage the optimal separation was achieved by two developments.
ASJC Scopus subject areas
- Molecular Medicine