Effect of ovariectomy and hormone replacement therapy on small artery biomechanics in angiotensin-induced hypertension in rats

S. Várbíró, G. Nádasy, E. Monos, Zoltán Vajoé, N. Ács, Zsuzsanna Miklós, A. Tőkés, Béla Székécs

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Objectives: To test the effects of chronic angiotensin II administration on blood pressure and small artery biomechanics in the female sex hormone-depleted state (proposed to increase cardiovascular vulnerability) and with hormone replacement. Design: Biomechanical properties of saphenous artery segments from ovariectomized (n = 10), ovariectomized + chronically angiotensin II infused-(n = 10), and ovariectomized + chronically angiotensin II-infused + sex hormone-replaced (n = 10) rats were studied. Methods: Surgical ovariectomy was performed. Osmotic minipumps were used for chronic angiotensin II infusion (100 ng/min per kg). For hormone replacement therapy, oestradiol-propionate, 450 μg/kg for 7 days + medroxyprogesterone-acetate, 15 mg/kg for 14 days were given, intramuscularly. After 4 weeks, cylindrical segments of the saphenous artery were prepared and subjected to in-vitro microarteriographic measurements. Pressure-diameter curves (0-200 mmHg) were recorded in Krebs-Ringer solution, with smooth muscle contracted (norepinephrine, 16 μmol/l) and with relaxed (papaverine, 28 μmol/l). Results: Chronic angiotensin II infusion significantly reduced the inner radius (at 100 mmHg: 298 ± 17 μm versus 347 ± 7 pm, P

Original languageEnglish
Pages (from-to)1587-1595
Number of pages9
JournalJournal of Hypertension
Volume18
Issue number11
Publication statusPublished - 2000

Fingerprint

Hormone Replacement Therapy
Angiotensins
Ovariectomy
Biomechanical Phenomena
Angiotensin II
Arteries
Hypertension
Gonadal Steroid Hormones
Papaverine
Medroxyprogesterone Acetate
Propionates
Smooth Muscle
Estradiol
Norepinephrine
Hormones
Blood Pressure
Pressure

Keywords

  • Angiotensin II
  • Estradiol
  • Hormone replacement therapy
  • Hypertension
  • Menopause
  • Progesterone
  • Small artery
  • Vascular resistance

ASJC Scopus subject areas

  • Internal Medicine
  • Endocrinology

Cite this

Effect of ovariectomy and hormone replacement therapy on small artery biomechanics in angiotensin-induced hypertension in rats. / Várbíró, S.; Nádasy, G.; Monos, E.; Vajoé, Zoltán; Ács, N.; Miklós, Zsuzsanna; Tőkés, A.; Székécs, Béla.

In: Journal of Hypertension, Vol. 18, No. 11, 2000, p. 1587-1595.

Research output: Contribution to journalArticle

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