Effect of nucleoside transport blockade on the interstitial adenosine level characterized by a novel method in guinea pig atria

Dénes Karsai, Judit Zsuga, B. Juhász, Péter Dér, András József Szentmiklósi, A. Tósaki, R. Gesztelyi

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Several accepted methods are available to estimate the adenosine (Ado) concentration of interstitial fluid ([Ado]ISF) in functioning heart, providing results spanning over nano- to micromolar concentrations. This extremely large range points to the necessity of novel approaches for estimating [Ado]ISF or at least the alteration from basal [Ado]ISF. In the present study, the change in [Ado]ISF was characterized following nucleoside transport (NT) blockade elicited by 10 μmol/L dipyridamole or 10 μmol/L nitrobenzylthioinosine in isolated guinea pig atria, by means of our novel procedure referred to as receptorial responsiveness method (RRM). The RRM provided an index of the change in [Ado]ISF under NT blockade, namely the concentration of N-cyclopentyladenosine (CPA; a relatively stable A1 Ado receptor agonist), which is equieffective with the change in [Ado]ISF regarding the contractility. Our results show that dipyridamole or nitrobenzylthioinosine produced an elevation in [Ado]ISF at the cardiomyocyte A1 Ado receptors equivalent to about 16 or 20 nmol/l CPA, respectively. In addition, nitrobenzylthioinosine was found more appropriate for selective NT blockade than dipyridamole.

Original languageEnglish
Pages (from-to)103-109
Number of pages7
JournalJournal of Cardiovascular Pharmacology
Volume47
Issue number1
DOIs
Publication statusPublished - Jan 2006

Fingerprint

Nucleosides
Adenosine
Guinea Pigs
Dipyridamole
Adenosine A1 Receptor Agonists
Adenosine A1 Receptors
Extracellular Fluid
Cardiac Myocytes

Keywords

  • Atrium
  • Guinea pig
  • Heart
  • Interstitial adenosine
  • Nucleoside transport
  • Receptorial responsiveness method

ASJC Scopus subject areas

  • Pharmacology
  • Cardiology and Cardiovascular Medicine

Cite this

Effect of nucleoside transport blockade on the interstitial adenosine level characterized by a novel method in guinea pig atria. / Karsai, Dénes; Zsuga, Judit; Juhász, B.; Dér, Péter; Szentmiklósi, András József; Tósaki, A.; Gesztelyi, R.

In: Journal of Cardiovascular Pharmacology, Vol. 47, No. 1, 01.2006, p. 103-109.

Research output: Contribution to journalArticle

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