Effect of noradrenaline and vanadium on Na+K+-activated ATPase in rat cerebral cortex synaptosomal preparation

V. Ádám-Vizi, Mária Ördögh, I. Horváth, J. Somogyi, E. Vízi

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

The effect of l-noradrenaline and vanadate on the activity of Na+K+-activated ATPase was studied on synaptosomal brain cortex preparation. Using neutron activation analysis it was found that the rat cerebral cortex synaptosomal preparation contains 0.16 μM. vanadium. The concentration of vanadium needed to reduce enzyme activity by 50% proved to be 2×10-6 M. Evidence has been provided that the increase by noradrenaline of enzyme activity in synaptosomal preparation depends on the presence of an inhibitory contaminant in commercial ATP preparations. In homogenate, however, noradrenaline was able to enhance enzyme activity even when vanadium-free ATP was used. This fact indicates that noradrenaline removes the inhibitory effect of cytoplasmic factor thereby stimulating enzyme activity.

Original languageEnglish
Pages (from-to)53-60
Number of pages8
JournalJournal of Neural Transmission
Volume47
Issue number1
DOIs
Publication statusPublished - Mar 1980

Fingerprint

Vanadium
Cerebral Cortex
Adenosine Triphosphatases
Norepinephrine
Enzymes
Adenosine Triphosphate
Neutron Activation Analysis
Vanadates
Brain

Keywords

  • NaK-activated ATPase
  • noradrenaline
  • synaptosome
  • vanadate

ASJC Scopus subject areas

  • Neuroscience(all)
  • Medicine(all)

Cite this

Effect of noradrenaline and vanadium on Na+K+-activated ATPase in rat cerebral cortex synaptosomal preparation. / Ádám-Vizi, V.; Ördögh, Mária; Horváth, I.; Somogyi, J.; Vízi, E.

In: Journal of Neural Transmission, Vol. 47, No. 1, 03.1980, p. 53-60.

Research output: Contribution to journalArticle

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