Effect of NO-donors, SIN-1 and GEA 3175 on prostacyclin and cGMP synthesis in cultured rat endothelial cells

J. Alanko, E. Sievi, T. Lahteenmaki, I. Muchá, A. Riutta, H. Vapaatalo

Research output: Contribution to journalArticle

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Abstract

The aim of the present study was to investigate, whether nitric oxide (NO) modifies prostacyclin synthesis in endothelial cells. Two different NO-donors: SIN-1 (3-morpholino sydnonimine) and GEA 3175 (4-aryl-substituted oxatriazol derivative), and the NO-synthesis inhibitor; L-NAME were used. Endothelial cells were incubated with the tested compounds with or without Ca ionophore A23187 stimulation. SIN-1 ( > 33 μM) and GEA 3175 ( > 1 μM) increased the endothelial cGMP levels independently of A23187 stimulation. SIN-1 did not influence prostacyclin synthesis. GEA 3175 ( > 33 μM) increased prostacyclin synthesis up to 2-fold, when incubated without A23187. GEA 3175 with A23187 induced about 30% inhibition in prostacyclin synthesis. L-NAME decreased unstimulated prostacyclin synthesis and this inhibition was reversed by GEA 3175. Obviously NO is able to modulate prostacyclin synthesis, however, much higher concentrations are needed than those to increase cGMP synthesis.

Original languageEnglish
Pages (from-to)195-199
Number of pages5
JournalAgents and Actions
Volume45
Issue numberSUPPL. I
Publication statusPublished - 1995

Fingerprint

Nitric Oxide Donors
Endothelial cells
Epoprostenol
Rats
Endothelial Cells
Calcimycin
Nitric Oxide
NG-Nitroarginine Methyl Ester
Ionophores
GEA 3175
Derivatives

ASJC Scopus subject areas

  • Pharmacology (medical)
  • Toxicology

Cite this

Alanko, J., Sievi, E., Lahteenmaki, T., Muchá, I., Riutta, A., & Vapaatalo, H. (1995). Effect of NO-donors, SIN-1 and GEA 3175 on prostacyclin and cGMP synthesis in cultured rat endothelial cells. Agents and Actions, 45(SUPPL. I), 195-199.

Effect of NO-donors, SIN-1 and GEA 3175 on prostacyclin and cGMP synthesis in cultured rat endothelial cells. / Alanko, J.; Sievi, E.; Lahteenmaki, T.; Muchá, I.; Riutta, A.; Vapaatalo, H.

In: Agents and Actions, Vol. 45, No. SUPPL. I, 1995, p. 195-199.

Research output: Contribution to journalArticle

Alanko, J, Sievi, E, Lahteenmaki, T, Muchá, I, Riutta, A & Vapaatalo, H 1995, 'Effect of NO-donors, SIN-1 and GEA 3175 on prostacyclin and cGMP synthesis in cultured rat endothelial cells', Agents and Actions, vol. 45, no. SUPPL. I, pp. 195-199.
Alanko, J. ; Sievi, E. ; Lahteenmaki, T. ; Muchá, I. ; Riutta, A. ; Vapaatalo, H. / Effect of NO-donors, SIN-1 and GEA 3175 on prostacyclin and cGMP synthesis in cultured rat endothelial cells. In: Agents and Actions. 1995 ; Vol. 45, No. SUPPL. I. pp. 195-199.
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AU - Sievi, E.

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AU - Riutta, A.

AU - Vapaatalo, H.

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AB - The aim of the present study was to investigate, whether nitric oxide (NO) modifies prostacyclin synthesis in endothelial cells. Two different NO-donors: SIN-1 (3-morpholino sydnonimine) and GEA 3175 (4-aryl-substituted oxatriazol derivative), and the NO-synthesis inhibitor; L-NAME were used. Endothelial cells were incubated with the tested compounds with or without Ca ionophore A23187 stimulation. SIN-1 ( > 33 μM) and GEA 3175 ( > 1 μM) increased the endothelial cGMP levels independently of A23187 stimulation. SIN-1 did not influence prostacyclin synthesis. GEA 3175 ( > 33 μM) increased prostacyclin synthesis up to 2-fold, when incubated without A23187. GEA 3175 with A23187 induced about 30% inhibition in prostacyclin synthesis. L-NAME decreased unstimulated prostacyclin synthesis and this inhibition was reversed by GEA 3175. Obviously NO is able to modulate prostacyclin synthesis, however, much higher concentrations are needed than those to increase cGMP synthesis.

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