Effect of nitrogen forms on growth, cell composition and N2 fixation of Cylindrospermopsis raciborskii in phosphorus-limited chemostat cultures

Gyöngyi Kenesi, Hesham M. Shafik, Attila W. Kovács, Sándor Herodek, M. Présing

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

The aim of this research was to test whether NH4 + and NO3 - affect the growth, P demand, cell composition and N2 fixation of Cylindrospermopsis raciborskii under P limitation. Experiments were carried out in P-limited (200 μg l-1 PO 4-P) chemostat cultures of C. raciborskii using an inflowing medium containing either 4,000 μg l-1 NH4-N, 4,000 μg l-1 NO3-N or no combined N. The results showed the cellular N:P and C:P ratios of C. raciborskii decreased towards the Redfield ratio with increasing dilution rate (D) due to the alleviation of P limitation. The cellular C:N and carotenoids:chlorophyll-a ratios also decreased with D, predominantly as a result of an increase in the chlorophyll-a and N content. The NH4 + and NO3 - supply reduced the P maintenance cell quota of C. raciborskii. Consequently, the biomass yield of the N2-grown culture was significantly lower. The maximum specific growth rate of N2-grown culture was also the lowest observed. It is suggested that these differences in growth parameters were caused by the P and energy requirement for heterocyte formation, nitrogenase synthesis and N 2 fixation. N2 fixation was partially inhibited by NO 3 - and completely inhibited by NH4 +. It was probably repressed through the high N content of cells at high dissolved N concentrations. These results indicate that C. raciborskii is able to grow faster and maintain a higher biomass under P limitation where a sufficient supply of NH4 + or NO3 - is maintained. Information gained about the species-specific nutrient and pigment stoichiometry of C. raciborskii could help to access the degree of nutrient limitation in water bodies.

Original languageEnglish
Pages (from-to)191-202
Number of pages12
JournalHydrobiologia
Volume623
Issue number1
DOIs
Publication statusPublished - May 2009

Fingerprint

Cylindrospermopsis raciborskii
chemostat
fixation
cell growth
phosphorus
nitrogen
chlorophyll a
Redfield ratio
nutrient limitation
biomass
stoichiometry
carotenoid
pigment
dilution
chlorophyll
nutrient
nitrogenase
nutrients
cells
energy requirements

Keywords

  • Chemostat
  • Cylindrospermopsis raciborskii
  • Heterocytic cyanobacteria
  • N fixation
  • Phosphorus limitation

ASJC Scopus subject areas

  • Aquatic Science

Cite this

Effect of nitrogen forms on growth, cell composition and N2 fixation of Cylindrospermopsis raciborskii in phosphorus-limited chemostat cultures. / Kenesi, Gyöngyi; Shafik, Hesham M.; Kovács, Attila W.; Herodek, Sándor; Présing, M.

In: Hydrobiologia, Vol. 623, No. 1, 05.2009, p. 191-202.

Research output: Contribution to journalArticle

Kenesi, Gyöngyi ; Shafik, Hesham M. ; Kovács, Attila W. ; Herodek, Sándor ; Présing, M. / Effect of nitrogen forms on growth, cell composition and N2 fixation of Cylindrospermopsis raciborskii in phosphorus-limited chemostat cultures. In: Hydrobiologia. 2009 ; Vol. 623, No. 1. pp. 191-202.
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AB - The aim of this research was to test whether NH4 + and NO3 - affect the growth, P demand, cell composition and N2 fixation of Cylindrospermopsis raciborskii under P limitation. Experiments were carried out in P-limited (200 μg l-1 PO 4-P) chemostat cultures of C. raciborskii using an inflowing medium containing either 4,000 μg l-1 NH4-N, 4,000 μg l-1 NO3-N or no combined N. The results showed the cellular N:P and C:P ratios of C. raciborskii decreased towards the Redfield ratio with increasing dilution rate (D) due to the alleviation of P limitation. The cellular C:N and carotenoids:chlorophyll-a ratios also decreased with D, predominantly as a result of an increase in the chlorophyll-a and N content. The NH4 + and NO3 - supply reduced the P maintenance cell quota of C. raciborskii. Consequently, the biomass yield of the N2-grown culture was significantly lower. The maximum specific growth rate of N2-grown culture was also the lowest observed. It is suggested that these differences in growth parameters were caused by the P and energy requirement for heterocyte formation, nitrogenase synthesis and N 2 fixation. N2 fixation was partially inhibited by NO 3 - and completely inhibited by NH4 +. It was probably repressed through the high N content of cells at high dissolved N concentrations. These results indicate that C. raciborskii is able to grow faster and maintain a higher biomass under P limitation where a sufficient supply of NH4 + or NO3 - is maintained. Information gained about the species-specific nutrient and pigment stoichiometry of C. raciborskii could help to access the degree of nutrient limitation in water bodies.

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