Effect of nickel (Ni2+) on primary human ovarian granulosa cells in vitro

Csaba Révész, Z. Forgács, Péter Lázár, Szabolcs Mátyás, Klára Rajczy, Ferenc Krizsa, Artúr Bernard, István Gáti

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Human ovarian granulosa cells obtained from women undergoing in vitro fertilization were exposed to 15.6, 31.25, 62.5, 125, 250, 500, 1000 μM Ni2+ for 48 h. To determine the site of action of Ni2+, the granulosa cells were stimulated to produce progesterone (P) by using maximally stimulating amounts of human chorionic gonadotropin (0.1 IU/ml hCG) or dibutyryl cyclic adenosine monophosphate (1 mM db-cAMP). The luteinizing hormone (LH) analog hCG was chosen because resultant P production requires an intact membrane receptor and db-cAMP was used to test for post LH receptor defects caused by Ni2+. Progesterone content of the culture medium was determined by radioimmunoassay (RIA), and viability of the cells was measured by MTT (3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) reduction test. Concentration-dependent depression in both hGC and db-cAMP stimulated P production was seen at 15.625 μM or higher concentration of Ni2+, which is not cytotoxic on human ovarian granulosa cells. The viability of cells was unaffected up to 31.25 μM and decreased significantly at 62.5 μM. Our results show a dose-related depression in stimulated P production of granulosa cells at a dose that does not induce significant cytotoxic action. These data indicate that the effect of Ni2+ on P production is not due to cytotoxicity, and the cellular site(s) of inhibitory action appears to be subsequent to the membrane receptor and production of cAMP.

Original languageEnglish
Pages (from-to)287-292
Number of pages6
JournalToxicology Mechanisms and Methods
Volume14
Issue number5
DOIs
Publication statusPublished - Sep 2004

Fingerprint

Granulosa Cells
Nickel
Cyclic AMP Receptors
Progesterone
Cell Survival
LH Receptors
Membranes
Cells
Chorionic Gonadotropin
Fertilization in Vitro
Luteinizing Hormone
Cyclic AMP
Radioimmunoassay
Culture Media
Cytotoxicity
In Vitro Techniques
Defects

Keywords

  • cAMP
  • Cytotoxicity
  • Granulosa cells
  • hCG
  • MTT
  • Nickel
  • Progesterone

ASJC Scopus subject areas

  • Toxicology
  • Health, Toxicology and Mutagenesis

Cite this

Effect of nickel (Ni2+) on primary human ovarian granulosa cells in vitro. / Révész, Csaba; Forgács, Z.; Lázár, Péter; Mátyás, Szabolcs; Rajczy, Klára; Krizsa, Ferenc; Bernard, Artúr; Gáti, István.

In: Toxicology Mechanisms and Methods, Vol. 14, No. 5, 09.2004, p. 287-292.

Research output: Contribution to journalArticle

Révész, C, Forgács, Z, Lázár, P, Mátyás, S, Rajczy, K, Krizsa, F, Bernard, A & Gáti, I 2004, 'Effect of nickel (Ni2+) on primary human ovarian granulosa cells in vitro', Toxicology Mechanisms and Methods, vol. 14, no. 5, pp. 287-292. https://doi.org/10.1080/15376520490434566
Révész, Csaba ; Forgács, Z. ; Lázár, Péter ; Mátyás, Szabolcs ; Rajczy, Klára ; Krizsa, Ferenc ; Bernard, Artúr ; Gáti, István. / Effect of nickel (Ni2+) on primary human ovarian granulosa cells in vitro. In: Toxicology Mechanisms and Methods. 2004 ; Vol. 14, No. 5. pp. 287-292.
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