Effect of neurotensin and immunneutralization with anti-neurotensin-serum on dopaminergic-cholinergic interaction in the striatum

András Töröcsik, Angelica Rakovska, T. Görcs, E. Vízi

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

The effect of neurotensin (NT) on the release of acetylcholine (ACh) and dopamine (DA) from striatal slices of the rat brain was studied. Neurotensin, 1-150 nM, was able to release ACh from cholinergic interneurons of the striatum. Like the response to electrical stimulation, the ACh-releasing effect of NT was completely inhibited by tetrodotoxin indicating that neuronal firing is involved in its effect. Immunneutrilization reduced the stimulation-evoked release of ACh, an effect that was much marked when the inhibitory dopaminergic input was suspended by sulpiride-selective antagonists of D2 receptors. Sulpiride, 0.1 mM, induced a 2-fold increase in the NT- and electrically-induced release of ACh. A quantitatively similar increase was also observed after degeneration of the nigrostriatal DA pathway with 6-hydroxydopamine (6-OHDA) (2 × 250 μg/animal, i.c.v.). However, the D2 receptor agonist quinpirole, 0.01 mM, significantly reduced the NT-induced release of ACh by 77%. Neurotensin enhanced the stimulation-evoked release of [3H]DA. These findings indicate that, using field stimulation when dopaminergic, cholinergic and NT-containing neurons are stimulated in concert, NT is capable of releasing both ACh and DA in the striatum, but its effect on ACh release is masked unless the D2 receptor-mediated tonic inhibitory effect of DA released from the nigro-striatal pathway is attenuated. Thus, in Parkinson's disease where the dopaminergic input is impaired, NT may be involved in producing cholinergic dominance.

Original languageEnglish
Pages (from-to)306-312
Number of pages7
JournalBrain Research
Volume612
Issue number1-2
DOIs
Publication statusPublished - May 28 1993

Fingerprint

Neurotensin
Cholinergic Agents
Acetylcholine
Serum
Dopamine
Corpus Striatum
Sulpiride
Oxidopamine
Quinpirole
Tetrodotoxin
Interneurons
Electric Stimulation
Parkinson Disease
Neurons

Keywords

  • Acetylcholine release
  • Dopamine release
  • Neurotensin
  • Presynaptic modulation
  • Striatum

ASJC Scopus subject areas

  • Developmental Biology
  • Molecular Biology
  • Clinical Neurology
  • Neuroscience(all)

Cite this

Effect of neurotensin and immunneutralization with anti-neurotensin-serum on dopaminergic-cholinergic interaction in the striatum. / Töröcsik, András; Rakovska, Angelica; Görcs, T.; Vízi, E.

In: Brain Research, Vol. 612, No. 1-2, 28.05.1993, p. 306-312.

Research output: Contribution to journalArticle

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