Effect of Na3VO4 and membrane potential on the structure of sarcoplasmic reticulum membrane

Troy J. Beeler, Laszlo Dux, Anthony N. Martonosi

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6 Citations (Scopus)

Abstract

Two-dimensional crystalline arrays of Ca2+-ATPase molecules develop after treatment of sarcoplasmic reticulum vesicles with Na3VO4 in a Ca2+-free medium. The influence of membrane potential upon the rate of crystallization was studied by ion substitution using oxonol VI and 3,3′-diethyl-2,2′-thiadicarbocyanine (Di-S-C2(5)) to monitor inside positive or inside negative membrane, potentials, respectively. Positive transmembrane potential accelerates the rate of crystallization of Ca2+-ATPase, while negative potential disrupts preformed Ca2+-ATPase crystals, suggesting an influence of transmembrane potential upon the conformation of Ca2+-ATPase.

Original languageEnglish
Pages (from-to)73-79
Number of pages7
JournalThe Journal of Membrane Biology
Volume78
Issue number1
DOIs
Publication statusPublished - Feb 1 1984

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Keywords

  • Ca transport
  • Ca, Mg-ATPase
  • crystals
  • membrane potential
  • sarcoplasmic reticulum
  • vanadate

ASJC Scopus subject areas

  • Biophysics
  • Physiology
  • Cell Biology

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