Effect of nano-sized, elemental selenium supplement on the proteome of chicken liver

G. Gulyas, E. Csosz, J. Prokisch, A. Javor, M. Mezes, M. Erdelyi, K. Balogh, T. Janaky, Z. Szabo, A. Simon, L. Czegledi

Research output: Contribution to journalArticle

8 Citations (Scopus)


The nano-sized (100–500 nm) selenium has higher bioavailability and relatively lower toxicity compared to other selenium forms. The objective of the present study was to compare liver proteome profiles of broiler chicken fed with control diet without Se supplementation and diet supplemented with nano-Se with 4.25 mg/kg DM. Differential proteome analyses were performed by two-dimensional gel electrophoresis (2D-PAGE) followed by tryptic digestion and protein identification by liquid chromatography–mass spectrometry (LC-MS). Seven hundred and eight spots were detected, and 18 protein spots showed significant difference in their intensity (p < 0.05) between the two groups. In response to nano-Se supplementation, the expression of 8 proteins was higher, and 5 proteins were lower in nano-Se supplemented group compared to control group. The functions of the differentially expressed proteins indicate that the high dose of selenium supplementation induced a dietary stress. Selenium supplementation may influence the metabolism of fatty acids and carbohydrates and antioxidant system, and increase the quantity of cytoskeletal actin and the expression of actin regulatory protein as well.

Original languageEnglish
Pages (from-to)502-510
Number of pages9
JournalJournal of Animal Physiology and Animal Nutrition
Issue number3
Publication statusPublished - Jun 2017


  • Two-Dimensional Polyacrylamide Gel Electrophoresis
  • chicken
  • liver
  • protein expression
  • selenium

ASJC Scopus subject areas

  • Food Animals
  • Animal Science and Zoology

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