Effect of multivitamins in an effervescent preparation on the respiratory burst of peritoneal macrophages in mice

J. Jakus, Tamás Kriska, Rozália Vanyür

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

The effect of regular intake of low doses of an effervescent multivitamin preparation on the free-radical-producing activity of murine peritoneal macrophages under conditions resembling a possible infection was studied in vitro. Initially, several groups of mice were fed a basal diet and given, for 2 weeks, water without or with supplementation of either α-tocopherol, ascorbic acid, riboflavin or a multivitamin preparation. The supplementation period was followed by a 2-week wash-out time interval during which control and multivitamin groups received deionized water. Macrophage stimulation tests using chemiluminescent spectroscopy were performed at the end of the supplementation and wash-out periods to determine cell counts and their capacity to produce free radicals. Multivitamin supplementation increased the number, and the reactive oxygen species-producing activity, of macrophages. This effect persisted for 2 weeks after higher dose of supplementation were stopped. Multivitamin supplementation lowered the steady-state radical concentrations of liver and spleen as measured by electron paramagnetic resonance spectroscopy. It also increased the antioxidant reactivity of the same organs, while there was no effect on the free radical concentration and antioxidant capacity of the kidney and brain. When taken regularly, low doses of multivitamin supplementation may have a beneficial effect on the defence mechanisms of the organism.

Original languageEnglish
Pages (from-to)501-508
Number of pages8
JournalBritish Journal of Nutrition
Volume87
Issue number5
Publication statusPublished - 2002

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Respiratory Burst
Peritoneal Macrophages
Free Radicals
Spectrum Analysis
Antioxidants
Macrophages
Tocopherols
Riboflavin
Water
Electron Spin Resonance Spectroscopy
Ascorbic Acid
Reactive Oxygen Species
Spleen
Cell Count
Diet
Kidney
Control Groups
Liver
Brain
Infection

Keywords

  • Chemiluminescence
  • Reactive oxygen species
  • Respiratory burst
  • Vitamins

ASJC Scopus subject areas

  • Medicine (miscellaneous)

Cite this

Effect of multivitamins in an effervescent preparation on the respiratory burst of peritoneal macrophages in mice. / Jakus, J.; Kriska, Tamás; Vanyür, Rozália.

In: British Journal of Nutrition, Vol. 87, No. 5, 2002, p. 501-508.

Research output: Contribution to journalArticle

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