Effect of monoamine oxidase inhibitor on the nerve elements of the isolated cat ileum

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Abstract

The nerve cells and nerve terminals of the isolated cat ileum were studied by fluorescence and electron microscopy using glutaraldehyde formalin mixture and potassium permanganate as a fixative, after treatment with the monoamine oxidase inhibitor nialamide. Quantitative estimates on vesicle population and density were based on measurements of axon profile surfaces and vesicle size distributions. Cell bodies and fibres displaying a yellow fluorescence are found after nialamide treatment in the myenteric and submucous plexus as well as in the mucosa. The nerve cells can be divided into three groups according to their granular vesicle content. The first type of cells contains granular vesicles of 1200-1400 Å; the third type of cells, larger (1800-2400 Å in diameter) so called neurosecretory vesicles. In the second type of cells vesicles could not be observed. Number and size of the granular vesicles in the nerve terminals increased after nialamide treatment. In the fibres, different population of granular vesicles can be found after treatment. 30% of the vesicles remain granular in the fibres of the isolated ileum after potassium permanganate fixation. The granular vesicles 1200-1400 Å in diameter contain probably 5 hydroxytryptamine. The cells (third cell type) containing the largest vesicles (1800-2400 Å) are probably neurosecretory elements, related to 5 HT, but not really 5 HT ergic neurones. The close connection of their processes with blood vessels and their lack of presynaptic contacts to other nerve elements might point in this direction.

Original languageEnglish
Pages (from-to)249-263
Number of pages15
JournalActa Morphologica Academiae Scientiarum Hungaricae
Volume22
Issue number3-4
Publication statusPublished - 1974

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Monoamine Oxidase Inhibitors
Ileum
Nialamide
Cats
Potassium Permanganate
Serotonin
Neurons
Submucous Plexus
Myenteric Plexus
Fixatives
Glutaral
Population Density
Fluorescence Microscopy
Formaldehyde
Blood Vessels
Axons
Electron Microscopy
Mucous Membrane
Fluorescence
Population

ASJC Scopus subject areas

  • Anatomy

Cite this

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abstract = "The nerve cells and nerve terminals of the isolated cat ileum were studied by fluorescence and electron microscopy using glutaraldehyde formalin mixture and potassium permanganate as a fixative, after treatment with the monoamine oxidase inhibitor nialamide. Quantitative estimates on vesicle population and density were based on measurements of axon profile surfaces and vesicle size distributions. Cell bodies and fibres displaying a yellow fluorescence are found after nialamide treatment in the myenteric and submucous plexus as well as in the mucosa. The nerve cells can be divided into three groups according to their granular vesicle content. The first type of cells contains granular vesicles of 1200-1400 {\AA}; the third type of cells, larger (1800-2400 {\AA} in diameter) so called neurosecretory vesicles. In the second type of cells vesicles could not be observed. Number and size of the granular vesicles in the nerve terminals increased after nialamide treatment. In the fibres, different population of granular vesicles can be found after treatment. 30{\%} of the vesicles remain granular in the fibres of the isolated ileum after potassium permanganate fixation. The granular vesicles 1200-1400 {\AA} in diameter contain probably 5 hydroxytryptamine. The cells (third cell type) containing the largest vesicles (1800-2400 {\AA}) are probably neurosecretory elements, related to 5 HT, but not really 5 HT ergic neurones. The close connection of their processes with blood vessels and their lack of presynaptic contacts to other nerve elements might point in this direction.",
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