Effect of modification of membrane saccharides on hormonal imprinting in Tetrahymena.

P. Kovács, G. Csaba, Z. Darvas, G. Liszkay

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Concanavalin-A (Con-A) competes with insulin for the insulin binding sites of the receptor, an event resulting in overlapping bonds. If the saccharide components of the receptor are subjected to periodate treatment the binding of insulin decreases and so does the imprinting evoked by it. Simultaneously, the binding of Con-A increase immediately after the treatment and 24 hours following the insulin imprinting. This phenomenon indicates that though the two ligands (i.e. insulin and Con-A) overlap on the intact receptor, alterations in the saccharide component influence their binding though not in the same manner and not in the same direction. Periodate treatment, similarly as observed in mammalian lymphocyte cultures, enhanced the division of Tetrahymenas and the peak of Con-A binding occurred parallel to the peak of division after periodate treatment of equal duration. Periodate treatment disturbed not only the binding of Con-A but that of other lectins as well. It has been concluded that hormonal imprinting requires a physiological condition of the membrane and any disturbance of the membrane will lead to a decrease in the efficacy of imprinting.

Original languageEnglish
Pages (from-to)189-195
Number of pages7
JournalActa Physiologica Hungarica
Volume69
Issue number2
Publication statusPublished - 1987

Fingerprint

Tetrahymena
Concanavalin A
Insulin
Membranes
Lectins
Binding Sites
Lymphocytes
Ligands
metaperiodate

ASJC Scopus subject areas

  • Physiology

Cite this

Effect of modification of membrane saccharides on hormonal imprinting in Tetrahymena. / Kovács, P.; Csaba, G.; Darvas, Z.; Liszkay, G.

In: Acta Physiologica Hungarica, Vol. 69, No. 2, 1987, p. 189-195.

Research output: Contribution to journalArticle

@article{b0603c95394045619d5f60209e6fe85d,
title = "Effect of modification of membrane saccharides on hormonal imprinting in Tetrahymena.",
abstract = "Concanavalin-A (Con-A) competes with insulin for the insulin binding sites of the receptor, an event resulting in overlapping bonds. If the saccharide components of the receptor are subjected to periodate treatment the binding of insulin decreases and so does the imprinting evoked by it. Simultaneously, the binding of Con-A increase immediately after the treatment and 24 hours following the insulin imprinting. This phenomenon indicates that though the two ligands (i.e. insulin and Con-A) overlap on the intact receptor, alterations in the saccharide component influence their binding though not in the same manner and not in the same direction. Periodate treatment, similarly as observed in mammalian lymphocyte cultures, enhanced the division of Tetrahymenas and the peak of Con-A binding occurred parallel to the peak of division after periodate treatment of equal duration. Periodate treatment disturbed not only the binding of Con-A but that of other lectins as well. It has been concluded that hormonal imprinting requires a physiological condition of the membrane and any disturbance of the membrane will lead to a decrease in the efficacy of imprinting.",
author = "P. Kov{\'a}cs and G. Csaba and Z. Darvas and G. Liszkay",
year = "1987",
language = "English",
volume = "69",
pages = "189--195",
journal = "Physiology International",
issn = "2498-602X",
publisher = "Akademiai Kiado",
number = "2",

}

TY - JOUR

T1 - Effect of modification of membrane saccharides on hormonal imprinting in Tetrahymena.

AU - Kovács, P.

AU - Csaba, G.

AU - Darvas, Z.

AU - Liszkay, G.

PY - 1987

Y1 - 1987

N2 - Concanavalin-A (Con-A) competes with insulin for the insulin binding sites of the receptor, an event resulting in overlapping bonds. If the saccharide components of the receptor are subjected to periodate treatment the binding of insulin decreases and so does the imprinting evoked by it. Simultaneously, the binding of Con-A increase immediately after the treatment and 24 hours following the insulin imprinting. This phenomenon indicates that though the two ligands (i.e. insulin and Con-A) overlap on the intact receptor, alterations in the saccharide component influence their binding though not in the same manner and not in the same direction. Periodate treatment, similarly as observed in mammalian lymphocyte cultures, enhanced the division of Tetrahymenas and the peak of Con-A binding occurred parallel to the peak of division after periodate treatment of equal duration. Periodate treatment disturbed not only the binding of Con-A but that of other lectins as well. It has been concluded that hormonal imprinting requires a physiological condition of the membrane and any disturbance of the membrane will lead to a decrease in the efficacy of imprinting.

AB - Concanavalin-A (Con-A) competes with insulin for the insulin binding sites of the receptor, an event resulting in overlapping bonds. If the saccharide components of the receptor are subjected to periodate treatment the binding of insulin decreases and so does the imprinting evoked by it. Simultaneously, the binding of Con-A increase immediately after the treatment and 24 hours following the insulin imprinting. This phenomenon indicates that though the two ligands (i.e. insulin and Con-A) overlap on the intact receptor, alterations in the saccharide component influence their binding though not in the same manner and not in the same direction. Periodate treatment, similarly as observed in mammalian lymphocyte cultures, enhanced the division of Tetrahymenas and the peak of Con-A binding occurred parallel to the peak of division after periodate treatment of equal duration. Periodate treatment disturbed not only the binding of Con-A but that of other lectins as well. It has been concluded that hormonal imprinting requires a physiological condition of the membrane and any disturbance of the membrane will lead to a decrease in the efficacy of imprinting.

UR - http://www.scopus.com/inward/record.url?scp=0023069096&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0023069096&partnerID=8YFLogxK

M3 - Article

VL - 69

SP - 189

EP - 195

JO - Physiology International

JF - Physiology International

SN - 2498-602X

IS - 2

ER -