Effect of milk on surface properties of Staphylococcus aureus from bovine mastitis

Wubshet Mamo, F. Rozgonyi, Stellan Hjertén, Torkel Wadström

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

The surface hydrophobicity of cells of Staphylococcus aureus strains isolated from bovine mastitis grown on conventional agar and broth media was drastically reduced after incubation with bovine milk. Strains grown in high carbohydrate-high salt media yielded cells with reduced surface hydrophobicity compared to cells grown in conventional media, and adding bovine milk to minimal medium also yielded cells with reduced surface hydrophobicity, as determined by hydrophobic interaction chromatography and the salt aggregation test. Incubation of strains in milk and growth in a medium supplemented with bovine milk also significantly changed bacterial surface charge as determined by free-zone electrophoresis. Strains with high or with decreased adsorptive and aggregating properties did not produce surface capsule or slime. Heat treatment (60° C or 80° C) of the bacterial suspensions did not significantly change their adsorptive and aggregating properties.

Original languageEnglish
Pages (from-to)195-200
Number of pages6
JournalFEMS Microbiology Letters
Volume48
Issue number1-2
Publication statusPublished - Dec 1 1987

Fingerprint

Bovine Mastitis
Surface Properties
Hydrophobic and Hydrophilic Interactions
Staphylococcus aureus
Milk
Salts
Agar
Capsules
Electrophoresis
Chromatography
Suspensions
Hot Temperature
Carbohydrates
Growth

Keywords

  • Bovine milk
  • Mastitis
  • S. aureus
  • Surface charge
  • Surface hydrophobicity

ASJC Scopus subject areas

  • Genetics
  • Molecular Biology
  • Applied Microbiology and Biotechnology
  • Microbiology

Cite this

Effect of milk on surface properties of Staphylococcus aureus from bovine mastitis. / Mamo, Wubshet; Rozgonyi, F.; Hjertén, Stellan; Wadström, Torkel.

In: FEMS Microbiology Letters, Vol. 48, No. 1-2, 01.12.1987, p. 195-200.

Research output: Contribution to journalArticle

Mamo, Wubshet ; Rozgonyi, F. ; Hjertén, Stellan ; Wadström, Torkel. / Effect of milk on surface properties of Staphylococcus aureus from bovine mastitis. In: FEMS Microbiology Letters. 1987 ; Vol. 48, No. 1-2. pp. 195-200.
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