Effect of microenvironment on the fluorescence of 2-hydroxy-substituted Nile Red dye: A new fluorescent probe for the study of micelles

Krisztina Nagy, Sinem Göktürk, László Biczók

Research output: Contribution to journalArticle

45 Citations (Scopus)

Abstract

Absorption spectra and fluorescence properties of 2-hydroxy-substituted Nile Red (HONR) have been studied in various solvents and micelles. The solvent dependence of the rate constant of the internal conversion was found to exhibit two entirely distinct domains. Going from apolar toward polar solvents, first, the deceleration of the radiationless deactivation was observed due to the diminishing extent of the vibronic coupling between the neighboring S1 and S2 singlet excited states. After reaching a minimum, the rate of the transition to the ground state significantly increased in alcohols because of the efficient energy dissipation via intermolecular hydrogen bonding. The correlations of the excited-state energy, fluorescence lifetime, absorption, and fluorescence maxima with the ET(30) solvent polarity parameter were used to characterize the local environment of HONR in micelles. Fluorescence quenching studies provided further evidences for the differences in the binding site of the dye in cationic, anionic and nonionic micelles.

Original languageEnglish
Pages (from-to)8784-8790
Number of pages7
JournalJournal of Physical Chemistry A
Volume107
Issue number41
DOIs
Publication statusPublished - Oct 16 2003

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Micelles
Fluorescent Dyes
micelles
Coloring Agents
dyes
Fluorescence
fluorescence
probes
Excited states
Deceleration
internal conversion
deceleration
Electron transitions
deactivation
Ground state
excitation
Absorption spectra
Quenching
Rate constants
Energy dissipation

ASJC Scopus subject areas

  • Physical and Theoretical Chemistry

Cite this

Effect of microenvironment on the fluorescence of 2-hydroxy-substituted Nile Red dye : A new fluorescent probe for the study of micelles. / Nagy, Krisztina; Göktürk, Sinem; Biczók, László.

In: Journal of Physical Chemistry A, Vol. 107, No. 41, 16.10.2003, p. 8784-8790.

Research output: Contribution to journalArticle

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