Effect of magnesium on the growth and alkaloid production of hairy root cultures

H. Hank, I. László, I. Bálványos, L. Kursinszki, Gy Kovács, E. Szőke, E. Tóth

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

In vitro cultures of Atropa belladonna L. (Solanaceae) cultivated on a half dilution MS (Murashige and Skoog) medium containing 3% sucrose, was infected by microinjection with Agrobacterium rhizogenes strain R1601. The hairy roots formed within 2 months were isolated, subcultured on B5 (Gamborg) medium and selected from the point of view of growth and tropane alkaloid production. Determination of alkaloids was performed by HPLC methods. Atropine, scopolamine and 6-OH-hyoscyamine were identified and measured by this technique. We have studied the effect of MgSO4 on the growth and alkaloid production of genetically modified hairy roots as well.

Original languageEnglish
Pages (from-to)271-274
Number of pages4
JournalActa Horticulturae
Volume597
Publication statusPublished - Jan 30 2004

Fingerprint

atropine
alkaloids
magnesium
Atropa belladonna
tropane alkaloids
scopolamine
Rhizobium rhizogenes
Solanaceae
in vitro culture
sucrose
methodology

Keywords

  • Atropa belladonna
  • Hairy root cultures
  • Hyoscyamine
  • Magnesium
  • Scopolamine

ASJC Scopus subject areas

  • Horticulture

Cite this

Hank, H., László, I., Bálványos, I., Kursinszki, L., Kovács, G., Szőke, E., & Tóth, E. (2004). Effect of magnesium on the growth and alkaloid production of hairy root cultures. Acta Horticulturae, 597, 271-274.

Effect of magnesium on the growth and alkaloid production of hairy root cultures. / Hank, H.; László, I.; Bálványos, I.; Kursinszki, L.; Kovács, Gy; Szőke, E.; Tóth, E.

In: Acta Horticulturae, Vol. 597, 30.01.2004, p. 271-274.

Research output: Contribution to journalArticle

Hank, H, László, I, Bálványos, I, Kursinszki, L, Kovács, G, Szőke, E & Tóth, E 2004, 'Effect of magnesium on the growth and alkaloid production of hairy root cultures', Acta Horticulturae, vol. 597, pp. 271-274.
Hank H, László I, Bálványos I, Kursinszki L, Kovács G, Szőke E et al. Effect of magnesium on the growth and alkaloid production of hairy root cultures. Acta Horticulturae. 2004 Jan 30;597:271-274.
Hank, H. ; László, I. ; Bálványos, I. ; Kursinszki, L. ; Kovács, Gy ; Szőke, E. ; Tóth, E. / Effect of magnesium on the growth and alkaloid production of hairy root cultures. In: Acta Horticulturae. 2004 ; Vol. 597. pp. 271-274.
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