Effect of magnesium on essential oil formation of genetically transformed and non-transformed chamomile cultures

E. Szőke, Emöke Máday, Sandor A. Kiss, E. Lemberkovics, Éva Lemberkovics

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Objective: The importance of chamomile (Chamomilla recutita) is widely known in classical and folk medicine, with the largest group of its effective substances forming the essential oil (chamazulene, α-bisabolol, trans-β-farnesene, spathulenol, cis/trans-en-in-dicycloethers). The increasing need for plant-derived high quality drugs cannot be provided by their collection in the wilderness. Method A: To preserve the genome of Szabadkigyós wild type having high (-)-α-bisabolol content, we used biotechnological methods. Results: The roots of organized culture contained β-eudesmol, which we have identified in the intact roots. Our gas-chromatographic and mass-spectroscopic studies showed that sterile chamomile cultures generated the most important terpenoid and polyin compounds characteristics of the intact plant. We identified berkheyaradulene, geranyl-isovalerate and cedrol, as new components in these cultures. Magnesium (Mg) (370 and 740 mg/l MgSO4) has a positive effect on the growth of organized cultures and also on the quality and quantity of essential oil production. Method B: Another possible source of variants is available by the genetic transformation of organized cultures by infection with Agrobacterium rhisogenes. With this method, we cultivated chamomile infected by A4-Y clone and investigated the essential oil production by hairy root cultures cultivated on solid and liquid MS B-5 media. The main component of the essential oil of hairy root cultures was trans-β-farnesene. Results: We identified α-selinene, as a new component in these hairy roots. We studied the growth rate of A4-Y clone on the cited media, containing MgSO4 concentrations: 0; 185; 370 and 740 mg/l. The cultures grew most in medium containing 740 mg/l of MgSO4. Essential oil content was compared from hairy root cultures of different Mg containing media and measured by GC and GC-MS methods. Mg has a similar effect on hairy roots as on organized cultures.

Original languageEnglish
JournalJournal of the American College of Nutrition
Volume23
Issue number6
Publication statusPublished - Dec 2004

Fingerprint

Matricaria
Matricaria chamomilla
Volatile Oils
Magnesium
magnesium
essential oils
farnesene
Clone Cells
Wilderness
clones
Genetic Transformation
spathulenol
methodology
wilderness
Agrobacterium
Terpenes
plant characteristics
Traditional Medicine
genetic transformation
Growth

Keywords

  • Agrobacterium rhizogenes
  • Chamomile
  • Chamomilla recutita
  • Essential oil
  • Hairy root
  • Magnesium
  • Organized culture

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Food Science

Cite this

Effect of magnesium on essential oil formation of genetically transformed and non-transformed chamomile cultures. / Szőke, E.; Máday, Emöke; Kiss, Sandor A.; Lemberkovics, E.; Lemberkovics, Éva.

In: Journal of the American College of Nutrition, Vol. 23, No. 6, 12.2004.

Research output: Contribution to journalArticle

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AU - Lemberkovics, Éva

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