Effect of macroelements on the growth and lobeline production of Lobelia inflata L. hairy root cultures

I. Bálványos, É Szoke, L. Kursinszki

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Lobelia inflata L. contains piperidine alkaloids. The main alkaloid is the pharmacologically active lobeline. We have studied the effect of macroelements 2+ + 2+ (Mg2+, Na+ and Ca2+) on the growth and alkaloid production of hairy root cultures of L. inflata (clone 8009/f4). Macroelements influenced characteristically the linear growth and biomass formation of hairy roots. The greatest biomass formed in B5 media containing 1000 mg/l MgSO4. It was found that the highest amount of lobeline (39μg/g, dry weight) was reached at 75 mg/l CaCl2. The results suggest that the optimal nutrient mixture for the best lobeline production was the basal B5 medium (containing 150 mg/l CaCl2 150 mg/l NaH 2PO4) supplemented with 1000 mg/l MgSO4.

Original languageEnglish
Pages (from-to)245-251
Number of pages7
JournalActa Horticulturae
Volume597
DOIs
Publication statusPublished - Jan 30 2004

Keywords

  • Hairy root
  • Lobelia inflata
  • Lobeline
  • Macroelements

ASJC Scopus subject areas

  • Horticulture

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