Effect of low temperature storage on the alpha-tocopherol (vitamin E) content of human lipoproteins determined by high-performance liquid chromatography

A. Somogyi, Klara Rosta, Magdolna Herold, Z. Tulassay

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Plasma lipoproteins contain a great amount of a-tocopherol (Vitamin E). To track the metabolism of this antioxidant in the human organism a great number of blood samples should be analysed. In order to improve the time consuming work-up of samples, the effect of low temperature storage on the constancy of α-tocopherol concentration in plasma and freshly separated lipoproteins have been studied by high-performance liquid chromatography (HPLC). We have established that up to the 42nd day there is no change both in the total tocopherol concentration of plasma and in the tocopherol content of lipid fractions. The sum of a-tocopherol concentrations, measured in the lipid fractions, always returned the 99.0±1.11 % of the a-tocopherol concentration found in plasma. Under the storage conditions used this study, after six weeks the decrease of content in the high density lipoprotein (HDL) fraction was 26.6 % (p

Original languageEnglish
Pages (from-to)807-815
Number of pages9
JournalACH - Models in Chemistry
Volume137
Issue number5-6
Publication statusPublished - 2000

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Tocopherols
High performance liquid chromatography
alpha-Tocopherol
Vitamin E
Lipoproteins
Plasmas
Temperature
Lipids
HDL Lipoproteins
Metabolism
Blood
Antioxidants

ASJC Scopus subject areas

  • Chemistry(all)

Cite this

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abstract = "Plasma lipoproteins contain a great amount of a-tocopherol (Vitamin E). To track the metabolism of this antioxidant in the human organism a great number of blood samples should be analysed. In order to improve the time consuming work-up of samples, the effect of low temperature storage on the constancy of α-tocopherol concentration in plasma and freshly separated lipoproteins have been studied by high-performance liquid chromatography (HPLC). We have established that up to the 42nd day there is no change both in the total tocopherol concentration of plasma and in the tocopherol content of lipid fractions. The sum of a-tocopherol concentrations, measured in the lipid fractions, always returned the 99.0±1.11 {\%} of the a-tocopherol concentration found in plasma. Under the storage conditions used this study, after six weeks the decrease of content in the high density lipoprotein (HDL) fraction was 26.6 {\%} (p",
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AU - Somogyi, A.

AU - Rosta, Klara

AU - Herold, Magdolna

AU - Tulassay, Z.

PY - 2000

Y1 - 2000

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